栽培茶树居群遗传结构及其保护策略初步研究

	栽培茶树居群遗传结构及其保护策略初步研究; A preliminary study on the genetic structure and conservation strategies of tea tree populations
	林阳蕾
导师	高立志
摘要	Tea tree (Camellia sinensis (L.) O. Ktze.) belongs to Camellia sect.Thea in Theaceae, and is one of the most important crops in the world. As the origin of tea tree, the southwest China has an extremely rich genetic diversity of tea germplasm resources, containing a large number of genes related to disease resistance and stress resistance. In this study, 10 pairs of polymorphic EST-SSR markers were used to research the genetic diversity and relationships of Camellia sinensis var. assamica populations in western and southern Yunnan Province, which provide a foundation for the protection, development and utilization of tea germplasm resources. And to understand the molecular mechanism of tea tree resistance in ex situ conservation process, we also analyzed CPP gene family in Camellia sinensis. The main conclusions were as follows: (1) The results showed that the average genetic diversity of populations in the study was relatively low. A total of about 27 alleles were detected, with an average of 2.693, and the average of polymorphism information content was 0.38. Among 15 populations, the Shannon diversity index ranged from 0.465 to 0.776, with an average of 0.608; the observed heterozygosity was between 0.211-0.543, with an average of 0.326; the expected heterozygosity was between 0.276-0.440, with an average of 0.340. And the BLS and HPS population had the highest genetic diversity. Analysis of molecular variance(AMOVA), genetic differentiation coefficient(FST), and gene flow(Nm) analysis indicated that the genetic variance within the populations was obviously larger than that among populations, the genetic differentiation among populations was unobvious. Principal coordinates analysis(PCoA), population structure analysis and cluster analysis based on UPGMA showed that all populations in the Jinuo Mountain area were clustered into one group, the DZS group and the DJS group in Lincang were also grouped together. The bottleneck test showed that HJZ and JNS-ZX population might undergo bottleneck recently. And in situ conservation should take priority for the species complemented by ex situ conservation according to our result of genetic diversity and genetic structure. (2) In this study, we totally identified 7, 8, and 8 non-redundant CPP genes in genomes of three tea cultivars, including C. sinensis var. assamica ‘Yunkang-10’, C. sinensis var. sinensis ‘Biyun’ and C. sinensis var. sinensis ‘Shuchazao’. The phylogenetic analysis and structural analysis of CPP members in tea tree, Arabidopsis thaliana (L.) Heynh., rice and kiwifruit showed that all CPP genes could be divided into three groups, and group III could be divided into two subgroups III-A and III-B. It was shown that CPP members in the same group or subgroup usually had similar length, gene structure and conserved motifs. The Cis-acting element analysis and study of gene expression patterns in different tissues indicated that CPP genes were involved in tea tree growth, development and stre
	2020-08
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74153
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	林阳蕾. 栽培茶树居群遗传结构及其保护策略初步研究, A preliminary study on the genetic structure and conservation strategies of tea tree populations[D],2020.

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