蔗糖对甘肃马先蒿吸器发生的促进效应及相关生理机制初探

	蔗糖对甘肃马先蒿吸器发生的促进效应及相关生理机制初探; Promoting Effects of Sucrose on Haustoria Formation in Pedicularis kansuensis Maxim and Relevant Physiological Mechanism
	李艳梅
导师	李爱荣
摘要	Haustoria are characteristic organs for parasitic plants, marking the transformation of parasitic plants from autotrophic to parasitic state, which are essential for understanding the evolution of parasitic plants. Spontaneous haustorium that produced in the absence of host signals are thought to be an important feature of early evolution stage for parasitic plants. Our previous studies showed that Pedicularis kansuensis can form spontaneous and are strongly regulated by sucrose, but the related mechanisms how sucrose regulates enhancement of spontaneous formation are largely unknown. Understanding the effects and the physiological mechanism of sucrose on P. kansuensis spontaneous haustorium formation are meaningful to explore the structure and physiological regulation of haustorium, and reveal the evolution process of haustorium formation. In this thesis, P. kansuensis was used as the research materials, agar plate culture experiments were conducted to investigate the difference of sucrose on spontaneous haustorium and inducted haustorium、the effects of sucrose and hormones on haustorium formation and relevant physiological mechanism. The results showed that: 1. Comparation of spontaneous haustoria and induced-haustoria in P. kansuensis Spontaneous haustoria and DMBQ-induced haustoria in P. kansuensis are consistent in position of occurrence and structure, but they are different from host- induced haustoria. Host-induced haustoria form at the primary and lateral roots, and some haustoria with xylem bridges. In contrast, spontaneous haustoria and DMBQ- induced haustoria only form at lateral roots and without xylem bridges. 2. Effects of haustoria induction factors (HIFs) and sucrose on haustoria formation in P. kansuensis HIFs are effective on haustorium formation, but there are some differences among them. P. kansuensis was more sensitive on quinones as 4-hydroxybenzaldehyde, vanillin and syringaldehyde, which bear an aldehyde group on benzene ring. Amendment sucrose significantly improved haustorium-induction ability of HIFs compare to control in P. kansuensis. 3. Effects of host plant and sucrose on haustoria formation in P. kansuensis Sucrose enhanced haustorium-induction efficiency of host plant, with the number of haustoria per plant increased 5.7 and percentage of haustoria with xylem bridges upgraded 17.9%. 4. Effects of hormones and sucrose on haustoria formation in P. kansuensis Auxin significantly promoted haustoria formation in P. kansuensis, but abscisic acid and cytokinin had less effects. Sucrose could increase haustorium-inducting ability of auxin and abscisic acid, with percentage of plant with haustoria increased from 13.9 to 41.5% and 1.8% to 7.8%. 5. Physiological mechanism of sucrose promoted spontaneous haustorium formation in P. kansuensis Sucrose and mannitol both affected haustorium formation in P. kansuensis, but percentage of plant with haustoria by sucrose was more 2 times than mannitol at same osmotic concentrations,
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74150
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	李艳梅. 蔗糖对甘肃马先蒿吸器发生的促进效应及相关生理机制初探, Promoting Effects of Sucrose on Haustoria Formation in Pedicularis kansuensis Maxim and Relevant Physiological Mechanism[D],2020.

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