蓖麻种子特异表达基因和基因组印记的表观调控研究

	蓖麻种子特异表达基因和基因组印记的表观调控研究; Study on epigenetic regulation of seed-specific genes and genomic imprinting in castor bean
	韩冰
导师	刘爱忠
摘要	The study of seed development in flowering plants has been a core content in plant reproductive development. Increasing evidence demonstrates epigenetic modifications, including histone modifications and DNA methylation, plays crucial roles in the control of seed development by regulating seed-specific genes and imprinted genes. In dicotyledons, relevant researches were mainly carried out in Arabidopsis. However, Arabidopsis seed size is small and endosperm exists instantaneously, so it is not an ideal material for seed development research. Castor bean (Ricinus communis L.) has relatively large and persistent endosperm throughout seed development. And castor bean is one of the important inedible oilseed crops and its seed oil is widely used in industry, especially for producing lubricating oil and biodiesel, because of its high content of the unique fatty acid ricinoleic acid. Therefore, it is of great theoretical and practical values to explore the epigenetic regulatory mechanism of seed development in castor bean. In this study, we investigated the pattern of epigenetic modifications and effects of epigenetic modifications on seed-specific genes and imprinted genes in castor bean ZB306 and reciprocal crosses between ZB107 and ZB306 by ChIP-seq, DNA methylation sequencing and transcriptome sequencing. Main results and conclusions were summarized as follows: 1. Characteristics of seed-specific genes in castor bean. Based on the transcriptome of various tissues, we identified 1162 seed-specific genes. Furthermore, depending on the expression pattern of seed-specific genes during seed development, we identified 327 early-stage genes, 264 middle-stage genes and 87 late-stage genes. GO analysis showed that carbohydrate metabolism mainly occurred in the early stage, lipid metabolism mainly occurred in the middle stage and protein metabolism mainly occurred in the late stage. Some important TFs, such as LEC1, LEC2, ABI3, FUS3 and WRI1, were found in castor bean. In addition to TFs, a large number of enzymes for biosynthesis of triacylglycerols (such as FAH12) and ricin genes were also found in castor bean. 2. DNA methylation valleys (DMVs) were enriched in seed-specific genes. By investigating the distribution of DNA methylation on seed-specific genes, we found that there was no difference among different tissues, which suggested that seed-specific genes were not regulated by DNA methylation directly. Further analysis showed that the DNA methylation levels of seed-specific genes were low, and DMVs were enriched in these genes. We identified 358 seed DMV genes and some key genes regulating seed development, such as LEC1, LEC2, ABI3, WRI1 and FAH12, were located by DMVs, strongly suggested that DMVs may be involved in the regulation of seed-specific genes. 3. Histone modifications were correlated with gene expression. First, we established ChIP method suitable to castor bean seed. And then we investigated the chromatin landscapes of histone modificati
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74126
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	韩冰. 蓖麻种子特异表达基因和基因组印记的表观调控研究, Study on epigenetic regulation of seed-specific genes and genomic imprinting in castor bean[D],2020.

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