基于比较基因组学和转录组学解析四季草莓连续开花的分子基础

	基于比较基因组学和转录组学解析四季草莓连续开花的分子基础; Analyzing the molecular basis of the continuous flowering trait in the perpetual-flowering strawberry by comparative genomics and transcriptomics
	杜海媛
导师	朱安丹
摘要	The effect of photoperiod on flowering has been studied in detail in Oryza sativa, Arabidopsis, Rosa chinensis and a diploid strawberry, Fragaria vesca. The phenomenon that photoperiod affects flowering also occurs in octoploid cultivated strawberries Fragaria ananassa. Some varieties (perpetual-flowering strawberry) are photoperiod-insensitive, which can initiate floral buds and keep blossoming and fruiting both under long-day conditions in summer and short-day conditions in winter. Although this continuous flowering trait attracts much interests in strawberry breeding, our understanding of its molecular mechanism is still limited. Previous studies indicated that a dominant genetic locus (FaPFRU), which locates in a chromosome homology to the F. vesca chromosome Ⅳ , could potentially regulate the summer flowering trait. However, it is still unclear that FaPFRU is a single gene or a tightly linked gene cluster. In this study, we investigated the candidate genes in regulating continuous flowering by combining genetic map analysis, comparative genomic analysis, transcriptome and qRT-PCR analysis. The main results are summarized as follows: The comparative genomic analysis showed that the homologous gene FaFT of A. thaliana FT gene in F. ananassa had different copy number among varieties with different flowering habits. The FT gene in perpetual-flowering (PF) strawberry (three copies) had one more copy than that in seasonal-flowering (SF) strawberry 'Camarosa' (two copies), and one of the copies of SF strawberry 'Camarosa' was terminated prematurely due to sequence deletion. That is, there may be only one functional FT gene in SF strawberry 'Camarosa', but three in PF strawberry. For TFL1 gene, a key gene in regulating continuous flowering traits in R. chinensis and F. vesca, did not show copy number difference between SF strawberry and PF strawberry. It is speculated that FaFT gene may be involved in the regulation of continuous flowering III traits of PF strawberries, and the regulation mode of continuous flowering traits in octoploid strawberries may be different from that in diploid F. vesca. We performed transcriptome sequencing and analysis from the shoots and leaves of two SF and two PF strawberry cultivars in summer and winter, respectively. The Principal Component Analysis (PCA) results indicated that there exist significant genetic and expression differences in different types of strawberries. The gene differential expression analysis showed that there are more than 10,000 differentially expressed genes between shoots and leaves. Among those differentially expressed genes, there are more than 200 genes related to flowering, indicating that the continuous flowering traits in octoploid strawberries may be regulated by multiple genes. Among the 200 genes, the expression of FT gene were different between winter and summer, and between strawberry varieties with different flowering habits. The FT gene expression level of PF strawberry in summer
	2020-05
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74118
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	杜海媛. 基于比较基因组学和转录组学解析四季草莓连续开花的分子基础, Analyzing the molecular basis of the continuous flowering trait in the perpetual-flowering strawberry by comparative genomics and transcriptomics[D],2020.

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