苔藓特异新基因PpG3功能研究

	苔藓特异新基因PpG3功能研究; The research of molecular function of moss-specific gene PpG3
	董秀梅
导师	刘莉
摘要	The origin of new functional genes is an important source of evolutionary innovation of species. It not only affects the phenotypic evolution of organisms, but also plays a very important role in the adaptation of species to the environment and biological processes necessary for organisms. Mosses occupy a phylogentic key position as the first colonized plant that transit from aquatic to terrestrial environment, and evolve an upright growth structure formed by shoot, leaf and rhizoid, and variously adversity adaptability which is mostly different from aquatic plant. Thus, mosses are very suitable experimental materials to investigate the relationship between new traits and new genes, which arising during the evolution. In Physcomitrium patens (P. patens), about 27% genes are new genes whose function is unknown, among which PpG3 (Pp3c3_15679) is a moss-specifc new gene. Therefore, on the one hand, we analyzed the function and possible mechanism of PpG3 in the response to stress. On the other hand, we analyzed the possible functions of PpG3 in plant development. PpG3 is a moss-specific orphan gene in P. patens and mainly expressed during the gametophytic stage of the life cycle, suggesting that it may play an important role in gametophytic stage. In addition, PpG3 expression was rapidly induced by dehydration, exogenous abscisic acid (ABA), and NaCl treatments, suggesting that PpG3 may play an important role in gametophytic stress response. A PpG3-knockout mutant showed reduced dehydration-rehydration tolerance, and the phenotype could be rescued by exogenous ABA. Meanwhile, transgenic Arabidopsis lines exhibiting heterologous expression of PpG3 showed enhanced drought tolerance. This indicates that PpG3 positively respond to drought stress potentially by recruiting ABA signaling pathway, and the recruitment of ABA signaling pathway by PpG3 is possibly a common mechanism that confers drought tolerance among land plants. RNA sequencing (RNA-seq) and weighted gene coexpression network analysis (WGCNA) indicated that PpG3 mediates protein processing in the endoplasmic reticulum (ER) connecting ABA signaling and stress responses, and it may be a pathway that is partially dependent on and independent of the known ABA signal response pathway. In addition, phenotypic studies of the PpG3-ko mutant during P. patens development showed that the gametophyte of the PpG3-ko tended to develop towards higher classes of shoot branches. PpG3, NLS- PPG3-N and NLS- PPG3-C were used to rescue PpG3-ko respectively, and the results also indicated that PpG3 involved in the regulation of shoot branching of P. patens gametophyte, and the function of N-terminal and C-terminal of PpG3 in the regulation of shoot branching is antagonistic. Transgenic Arabidopsis lines exhibiting heterologous expression of PpG3, NLS- PPG3-N and NLS- PPG3-C also showed the regulatory function of shoot branching in Arabidopsis was consistent with that of P. patens. Further analysis of the developme
	2020-11
文献类型	学位论文
条目标识符	http://ir.kib.ac.cn/handle/151853/74117
专题	昆明植物所硕博研究生毕业学位论文
推荐引用方式 GB/T 7714	董秀梅. 苔藓特异新基因PpG3功能研究, The research of molecular function of moss-specific gene PpG3[D],2020.

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