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Chemical profiling and antidiabetic potency of Paeonia delavayi: Comparison between different parts and constituents
Huang,Qian; Chen,Ji-Jun; Pan,Yang; He,Xiao-Feng; Wang,Yuan; Zhang,Xue-Mei; Geng,Chang-An
2021
发表期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN0731-7085
卷号198页码:113998
摘要Paeonia delavayi (Paeoniaceae), an endemic plant mainly distributed in southwest China, is always used as the substitute of P. suffruticosa due to their morphological and pharmacological similarity. In the previous study, P. suffruticosa was revealed with antidiabetic potency, whereas the chemical difference and antidiabetic property between different parts of P. delavayi has not yet been studied. This paper was designed to clarify the chemical constituents and antidiabetic potency of P. delavayi by LCMS analysis and enzyme inhibition on alpha-glucosidase, PTP1B, TCPTP, and DPP4. By interpretation of their UV absorptions and MS fragmentations, and/or comparison with reference samples, 57 constituents comprising 15 flavonoids, 10 monoterpene glycosides, eight triterpenoids, seven galloyl glucose s, six N-containing compounds, five gallic acids, two acetophenones, and four other types of compounds were identified from the different parts of P. delavayi. Moreover, two new monoterpene aglycones (42 and 47) and one new noroleanane triterpenoid (51) were speculated by their MS/MS fragmentation rules. Principal component analysis (PCA) suggested the chemical resemblance between root core and root bark which could be well differentiated with the leaves and stems by their characteristic constituents (monoterpene glycosides, flavonoids, and acetophenones). All the four parts (200 mu g/mL) showed obvious inhibition on alpha-glucosidase and PTP1B (81.2%-98.5%), but moderate to weak inhibition on TCPTP and DPP4 (19.5%-34.9%). Nine compounds representing five main types of constituents in Paeonia plants were assayed for their antidiabetic effects, indicating flavonoids and triterpenoids were the main active substances regarding to the four enzymes. Luteolin displayed obvious activity on alpha-glucosidase, PTP1B, and TCPTP with IC50 values of 94.6, 136.3, and 157.3 mu M, and akebonic acid could inhibit alpha-glucosidase and PTP1B with IC50 values of 73.5 and 57.8 mu M. Luteolin and ake-bonic acid were recognized as competitive inhibitors of alpha-glucosidase, but anticompetitive and mix-type inhibitors of PTP1B, respectively. Docking study demonstrated akebonic acid as PTP1B (over TCPTP) selective inhibitor by bonding to the catalytic sites (B/C) of PTP1B. This LCMS combined with enzymatic comparison opens new sights for recognizing the chemical profiles and antidiabetic potency of P. delavayi. (C) 2021 Elsevier B.V. All rights reserved.
关键词Paeonia delavayi Chemical comparison LCMS analyses Antidiabetic effects PTP1B/TCPTP selective inhibitors LIQUID-CHROMATOGRAPHY MULTIPLE CONSTITUENTS RAPID IDENTIFICATION GLYCOSIDES ROOT DERIVATIVES TARGETS CAPSULE L.
DOI10.1016/j.jpba.2021.113998
WOS记录号WOS:000636654000017
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/73677
专题中国科学院昆明植物研究所
作者单位1.[Huang, Qian
2.Chen, Ji-Jun
3.Pan, Yang
4.He, Xiao-Feng
5.Wang, Yuan
6.Zhang, Xue-Mei
7.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
8.Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
9.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Huang,Qian,Chen,Ji-Jun,Pan,Yang,et al. Chemical profiling and antidiabetic potency of Paeonia delavayi: Comparison between different parts and constituents[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2021,198:113998.
APA Huang,Qian.,Chen,Ji-Jun.,Pan,Yang.,He,Xiao-Feng.,Wang,Yuan.,...&Geng,Chang-An.(2021).Chemical profiling and antidiabetic potency of Paeonia delavayi: Comparison between different parts and constituents.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,198,113998.
MLA Huang,Qian,et al."Chemical profiling and antidiabetic potency of Paeonia delavayi: Comparison between different parts and constituents".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 198(2021):113998.
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