Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato

doi:10.1016/j.pld.2020.06.001

KIB OpenIR

	Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato
	Lin,Yuanyuan ; Huang,Junchao
	2021
发表期刊	PLANT DIVERSITY
ISSN	2096-2703
卷号	43 期号:1 页码:63-70
摘要	Establishing a transgenic plant largely relies on a selectable marker gene that can confer antibiotic or herbicide resistance to plant cells. The existence of such selectable marker genes in genetically modified foods has long been criticized. Plant cells generally exhibit too low an activity of phosphomannose isomerase (PMI) to grow with mannose as a sole carbon source. In this study, we characterized PMI from the green microalga Chlorococcum sp. and assessed its feasibility as a selectable marker for plant biotechnology. Chlorococcum sp. PMI (ChlPMI) was shown to be closely related to higher plants but more distant to bacterial counterparts. Overexpression of ChlPMI in tomato induced callus and shoot formation in media containing mannose (6 g/L) and had an average transformation rate of 3.9%. Based on this transformation system, a polycistronic gene cluster containing crtB, HpBHY, CrBKT and SlLCYB (BBBB) was co-expressed in a different tomato cultivar. Six putative transformants were achieved with a transformation rate of 1.4%, which produced significant amounts of astaxanthin due to the expression of the BBBB genes. Taken together, these findings indicate that we have established an additional tool for plant biotechnology that may be suitable for genetically modifying foods safely. Copyright (C) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
关键词	Algae Tomato Transformation Phosphomannose isomerase Astaxanthin SELECTION SYSTEM TRANSGENIC PLANTS ALTERNATIVES TRANSFORMATION ASTAXANTHIN EXPRESSION STRESS INDICA GROWTH
DOI	10.1016/j.pld.2020.06.001
WOS记录号	WOS:000630089100007
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73630
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lin,Yuanyuan,Huang,Junchao. Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato[J]. PLANT DIVERSITY,2021,43(1):63-70.
APA	Lin,Yuanyuan,&Huang,Junchao.(2021).Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato.PLANT DIVERSITY,43(1),63-70.
MLA	Lin,Yuanyuan,et al."Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato".PLANT DIVERSITY 43.1(2021):63-70.