Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit

doi:10.1007/s12374-021-09316-3

KIB OpenIR

	Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit
	Lin,Yuanyuan ; He,Mingxia; Wang,Jielin ; Huang,Junchao
	2021
发表期刊	JOURNAL OF PLANT BIOLOGY
ISSN	1226-9239
卷号	64 期号:5 页码:447-459
摘要	Tomato is a major source of dietary carotenoids that play an important role in human health. Improving the carotenoid profiles of tomato fruit has become a primary aspect in tomato breeding. The tomato 'Huang Song' landrace grows vigorously but with low contents of carotenoids in this yellowish fruit. To overcome this problem and extend lycopene to high-value astaxanthin, we co- expressed four genes encoding for phytoene synthase (crtB), beta-carotene hydroxylase (BHY), beta-carotene ketolase (BKT), and lycopene beta-cyclase (LCYB), respectively, in the fruit of the landrace via linking the genes with 2A-sequences and driving their expression with the E8 promoter. The transgenic lines showed similar phenotypes to WT except for the fruit colors. The transgenic fruit exhibited deep red color due to the accumulation of novel ketocarotenoids including astaxanthin, canthaxanthin, and ketolutein and enhanced production of native carotenoids which were 20 similar to 44-fold of the control. The T0, T1, and T2 plants exhibited similar growing status and carotenoid profiles, indicating that the transformants were genetically stable. The transgenes resulted in upregulation of most of the endogenous carotenogenic genes in fruit at mature green stage and more plastoglobules in chromoplast. This study provides insights into metabolic engineering of tomato for enhanced production of value-added carotenoids.
关键词	Tomato E8 promoter Carotenoid Lycopene Astaxanthin PHYTOENE SYNTHASE BETA-CAROTENE FLESH COLOR PLANTS ACCUMULATION METABOLISM ACID GENE BIOSYNTHESIS ASTAXANTHIN
DOI	10.1007/s12374-021-09316-3
WOS记录号	WOS:000659049500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73629
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lin,Yuanyuan,He,Mingxia,Wang,Jielin,et al. Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit[J]. JOURNAL OF PLANT BIOLOGY,2021,64(5):447-459.
APA	Lin,Yuanyuan,He,Mingxia,Wang,Jielin,&Huang,Junchao.(2021).Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit.JOURNAL OF PLANT BIOLOGY,64(5),447-459.
MLA	Lin,Yuanyuan,et al."Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit".JOURNAL OF PLANT BIOLOGY 64.5(2021):447-459.