Rubinoboletus ballouii polysaccharides exhibited immunostimulatory activities through toll-like receptor-4 via NF-kappa B pathway

doi:10.1002/ptr.6958

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	Rubinoboletus ballouii polysaccharides exhibited immunostimulatory activities through toll-like receptor-4 via NF-kappa B pathway
	Li,Long-Fei ; Yue,Grace G-L ; Chan,Ben C-L ; Zeng,Qiang ; Han,Quan-Bin ; Leung,Ping-Chung ; Fung,Kwok-Pui ; Liu,Ji-Kai ; Lau,Clara B-S
	2021
发表期刊	PHYTOTHERAPY RESEARCH
ISSN	0951-418X
卷号	35 期号:4 页码:2108-2118
摘要	The biological activities of water-soluble components of edible mushroom Rubinoboletus ballouii (RB) were seldom reported. Polysaccharides of RB (RBP) were prepared and well-characterized using chemical analyses. The immunomodulatory properties of RBP were investigated using human monocyte-derived dendritic cells (moDC) in vitro, and cyclophosphamide (CTX)-induced immunosuppressive mouse model. Results showed that RBP was found to contain 80.6% (w/w) of neutral sugars including D-fucose, D-mannose, D-glucose and D-galactose (1.7:1.4:1.0:1.8), and 12.5% (w/w) of proteins, which composed of glutamine, threonine, serine, etc. RBP could promote the maturation of moDC and increase the secretion of IL-12p40, IL-10, and TNF-alpha. Furthermore, the stimulation of IL-12p40 production was inhibited by pretreatment with toll-like receptor (TLR)-4 blocker or NF-kappa B pathway blocker, suggesting that the activation of moDC by RBP was mediated through NF-kappa B pathway via TLR-4 receptor. On the other hand, in CTX-treated mice, RBP restored the loss of CD34(bright)CD45(dim) hematopoietic stem cells and increased IL-2 production in sera and splenocytes culture supernatant, as well as up-regulated the percentage of CD4(+) T helper lymphocyte in mice splenocytes. These findings strongly suggested that RBP are the active ingredients of RB responsible for its immunostimulatory actions and deserved to be further investigated as cancer supplements.
关键词	immunostimulatory effects immunosuppression myelosuppression polysaccharide Rubinoboletus ballouii toll‐ like receptor‐ 4 GANODERMA-LUCIDUM DENDRITIC CELLS IN-VITRO ANTITUMOR MUSHROOMS
DOI	10.1002/ptr.6958
WOS记录号	WOS:000591447400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73552
专题	中国科学院昆明植物研究所
作者单位	1.[Li, Long-Fei 2.Yue, Grace G-L 3.Chan, Ben C-L 4.Zeng, Qiang 5.Leung, Ping-Chung 6.Fung, Kwok-Pui 7.Chinese Univ Hong Kong, Inst Chinese Med, Shatin, Hong Kong, Peoples R China 8.Chinese Univ Hong Kong, State Key Lab Res Bioact & Clin Applicat Med Plan, Shatin, Hong Kong, Peoples R China 9.Shenzhen Zhongshan Urol Hosp, Shenzhen Zhongshan Inst Reprod & Genet, Shenzhen Key Lab Reprod Immunol Peri Implantat, Shenzhen, Peoples R China 10.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Lab Mol Pharmacol, Luzhou, Peoples R China 11.Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China 12.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China 13.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 14.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Li,Long-Fei,Yue,Grace G-L,Chan,Ben C-L,et al. Rubinoboletus ballouii polysaccharides exhibited immunostimulatory activities through toll-like receptor-4 via NF-kappa B pathway[J]. PHYTOTHERAPY RESEARCH,2021,35(4):2108-2118.
APA	Li,Long-Fei.,Yue,Grace G-L.,Chan,Ben C-L.,Zeng,Qiang.,Han,Quan-Bin.,...&Lau,Clara B-S.(2021).Rubinoboletus ballouii polysaccharides exhibited immunostimulatory activities through toll-like receptor-4 via NF-kappa B pathway.PHYTOTHERAPY RESEARCH,35(4),2108-2118.
MLA	Li,Long-Fei,et al."Rubinoboletus ballouii polysaccharides exhibited immunostimulatory activities through toll-like receptor-4 via NF-kappa B pathway".PHYTOTHERAPY RESEARCH 35.4(2021):2108-2118.

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