Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation

doi:10.3390/ijms22042174

KIB OpenIR

	Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation
	Lin,Liang ; Ma,Junchao ; Ai,Qin ; Pritchard,Hugh W.; Li,Weiqi; Chen,Hongying
	2021
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	22 期号:4 页码:2174
摘要	Plant species conservation through cryopreservation using plant vitrification solutions (PVS) is based in empiricism and the mechanisms that confer cell integrity are not well understood. Using ESI-MS/MS analysis and quantification, we generated 12 comparative lipidomics datasets for membranes of embryogenic cells (ECs) of Magnolia officinalis during cryogenic treatments. Each step of the complex PVS-based cryoprotocol had a profoundly different impact on membrane lipid composition. Loading treatment (osmoprotection) remodeled the cell membrane by lipid turnover, between increased phosphatidic acid (PA) and phosphatidylglycerol (PG) and decreased phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PA increase likely serves as an intermediate for adjustments in lipid metabolism to desiccation stress. Following PVS treatment, lipid levels increased, including PC and PE, and this effectively counteracted the potential for massive loss of lipid species when cryopreservation was implemented in the absence of cryoprotection. The present detailed cryobiotechnology findings suggest that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS. As lipid metabolism and composition varies with species, these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction.
关键词	cryopreservation cryoprotectant agents ex situ conservation lipid Magnoliaceae plant vitrification solutions
DOI	10.3390/ijms22042174
WOS记录号	WOS:000623935100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73505
专题	中国科学院昆明植物研究所
作者单位	1.[Lin, Liang 2.Ma, Junchao 3.Ai, Qin 4.Pritchard, Hugh W. 5.Li, Weiqi 6.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 7.[Pritchard, Hugh W.] Royal Bot Gardens, Wellcome Trust Millennium Bldg,Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England
推荐引用方式 GB/T 7714	Lin,Liang,Ma,Junchao,Ai,Qin,et al. Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(4):2174.
APA	Lin,Liang,Ma,Junchao,Ai,Qin,Pritchard,Hugh W.,Li,Weiqi,&Chen,Hongying.(2021).Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(4),2174.
MLA	Lin,Liang,et al."Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.4(2021):2174.