Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities

doi:10.1016/j.ijbiomac.2021.07.049

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	Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities
	Ma,Yan ; Gao,Na ; Zuo,Zhichuang ; Li,Shanni ; Zheng,Wenqi ; Shi,Xiang ; Liu,Qipei ; Ma,Ting ; Yin,Ronghua ; Li,Xian ; Zhao,Jinhua
	2021
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	186 页码:535-543
摘要	Fucan sulfates from echinoderm possess characteristic structures and various biological activities. Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis. Chemical composition analysis showed that they were all composed of L-fucose. Their sulfate content was determined by a conductimetric method. The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively. Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was -> 3)-L-Fuc(S)-alpha(1 ->, where Fuc(S) were Fuc(2S4S) (10%), Fuc(2S) (44%), Fuc(0S) (10%), Fuc(4S) (36%), that of FSIII was -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(0S/3S)-(alpha 1 -> where Fuc(0S) and Fuc(3S) were in equal molar, and that FSIV was -> 4)-L-Fuc(2S3S)-(alpha 1 -> 4)-L-Fuc(2S3S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 ->. This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species. Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT. Among them, FSII, FSIII and FSIV showed the similar potency, while FSI owned the strongest. Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity.
关键词	Sea cucumber Fucan sulfate Chemical structure Anticoagulant Pattalus mollis FUCOSYLATED GLYCOSAMINOGLYCAN REPEATING UNIT FUCOIDAN POLYSACCHARIDES ELUCIDATION OLIGOSACCHARIDES SPECTROSCOPY
DOI	10.1016/j.ijbiomac.2021.07.049
WOS记录号	WOS:000688434600010
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73500
专题	中国科学院昆明植物研究所
作者单位	1.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China 2.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China 3.South Cent Univ Natl, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Ma,Yan,Gao,Na,Zuo,Zhichuang,et al. Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,186:535-543.
APA	Ma,Yan.,Gao,Na.,Zuo,Zhichuang.,Li,Shanni.,Zheng,Wenqi.,...&Zhao,Jinhua.(2021).Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,186,535-543.
MLA	Ma,Yan,et al."Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 186(2021):535-543.