Knowledge Management System of Kunming Institute of Botany,CAS
| Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves | |
Li,Xiong ; Chen,Di; Li,Boqun; Yang,Ya; Yang,Yongping
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| 2021 | |
| Source Publication | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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| ISSN | 0944-1344 |
| Volume | 28Issue:18Pages:22458-22473 |
| Abstract | Cadmium (Cd) pollution is a prominent environment problem, and great interests have been developed towards the molecular mechanism of Cd accumulation in plants. In this study, we conducted combined transcriptomic, proteomic and biochemical approaches to explore the detoxification of a Cd-hyperaccumulating turnip landrace exposed to 5 mu M (T5) and 25 mu M (T25) Cd treatments. A total of 1090 and 2111 differentially expressed genes (DEGs) and 161 and 303 differentially expressed proteins (DEPs) were identified in turnips under T5 and T25, respectively. However, poor correlations were observed in expression changes between mRNA and protein levels. The enriched KEGG pathways of DEGs with a high proportion (> 80%) of upregulated genes were focused on the flavonoid biosynthesis, sulphur metabolism and glucosinolate biosynthesis pathways, whereas those of DEPs were enriched on the glutathione metabolism pathway. This result suggests that these pathways contribute to Cd detoxification in turnips. Furthermore, induced antioxidant enzymes, heat stock proteins and stimulated protein acetylation modification seemed to play important roles in Cd tolerance in turnips. In addition, several metal transporters were found responsible for the Cd accumulation capacity of turnips. This study may serve as a basis for breeding low-Cd-accumulating vegetables for foodstuff or high-Cd-abstracting plants for phytoremediation. |
| Keyword | Turnip Cadmium Hyperaccumulator Flavonoid Acetylation RNA-SEQ DATA GENOME-WIDE IDENTIFICATION GLUTATHIONE METABOLISM SULFUR ASSIMILATION GENE FAMILIES STRESS EFFLUX ZINC ACCUMULATION PROTEIN |
| DOI | 10.1007/s11356-020-11454-z |
| WOS ID | WOS:000606176600005 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/73499 |
| Collection | 中国科学院昆明植物研究所 |
| Affiliation | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China |
| Recommended Citation GB/T 7714 | Li,Xiong,Chen,Di,Li,Boqun,et al. Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2021,28(18):22458-22473. |
| APA | Li,Xiong,Chen,Di,Li,Boqun,Yang,Ya,&Yang,Yongping.(2021).Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,28(18),22458-22473. |
| MLA | Li,Xiong,et al."Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 28.18(2021):22458-22473. |
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