USP47 maintains the stemness of colorectal cancer cells and is inhibited by parthenolide

doi:10.1016/j.bbrc.2021.05.017

KIB OpenIR

	USP47 maintains the stemness of colorectal cancer cells and is inhibited by parthenolide
	Zhang,Shaohua ; Ju,Xiaoman ; Yang,Qihong ; Zhu,Yiying ; Fan,Dongmei ; Su,Guifeng ; Kong,Lingmei ; Li,Yan
	2021
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	562 页码:21-28
摘要	Colorectal cancer stem cells (CCSCs) are implicated in colorectal tumor initiation, invasion, recurrence and treatment resistance, so elucidation of the mechanism underlying the cancer stem cells induction and development of drugs targeting CCSCs are vital for cancer treatment. Growing evidence shows that dysregulated deubiquitinase (DUBs) expression is frequently associated with stemness and maintenance of cancer stem cells (CSCs). In the current study, we found that upregulation of USP47 is associated with tumorigenesis and poor prognosis in clinical patients with colorectal cancer (CRC). Besides, USP47 was highly expressed in CCSCs enriched by serum-free culture. Further investigation showed that USP47 is closely involved in the maintenance of the stemness of CCSCs. USP47 silencing reduces proliferation and migration of colorectal cancer cells and suppresses the self-renewal of CCSCs by downregulating the expression of cancer stem cell markers, including CD44, CD133, CD166, OCT4 and NANOG. Furthermore, we identified Parthenolide (PTL), a natural sesquiterpene lactone, as a novel USP47 inhibitor. PTL diminishes CCSCs self-renewal and induces apoptosis of CCSCs. Taken together, our findings highlighted a novel DUB involved in the modulation of CCSCs stemness and the potential of PTL in the CRC treatment by targeting CCSCs as the USP47 inhibitor.(c) 2021 Elsevier Inc. All rights reserved.
关键词	USP47 Cancer stem cell Colorectal cancer Parthenolide Deubiquitinase SESQUITERPENE LACTONE PARTHENOLIDE DEUBIQUITINASES STABILIZATION LEUKEMIA
DOI	10.1016/j.bbrc.2021.05.017
WOS记录号	WOS:000653197100004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73496
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, 132 Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhang,Shaohua,Ju,Xiaoman,Yang,Qihong,et al. USP47 maintains the stemness of colorectal cancer cells and is inhibited by parthenolide[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2021,562:21-28.
APA	Zhang,Shaohua.,Ju,Xiaoman.,Yang,Qihong.,Zhu,Yiying.,Fan,Dongmei.,...&Li,Yan.(2021).USP47 maintains the stemness of colorectal cancer cells and is inhibited by parthenolide.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,562,21-28.
MLA	Zhang,Shaohua,et al."USP47 maintains the stemness of colorectal cancer cells and is inhibited by parthenolide".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 562(2021):21-28.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
132-H31-097.pdf（2615KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载