Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean

doi:10.1038/s41598-021-85644-1

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	Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean
	Wang,Zaiqing ; Yu,Anmin ; Li,Fei ; Xu,Wei; Han,Bing ; Cheng,Xiaomao ; Liu,Aizhong
	2021
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	11 期号:1 页码:6277
摘要	Plant dwarfism is a desirable agronomic trait in non-timber trees, but little is known about the physiological and molecular mechanism underlying dwarfism in woody plants. Castor bean (Ricinus communis) is a typical woody oilseed crop. We performed cytological observations within xylem, phloem and cambia tissues, revealing that divergent cell growth in all tissues might play a role in the dwarf phenotype in cultivated castor bean. Based on bulked segregant analyses for a F-2 population generated from the crossing of a tall and a dwarf accession, we identified two QTLs associated with plant height, covering 325 candidate genes. One of these, Rc5NG4-1 encoding a putative IAA transport protein localized in the tonoplast was functionally characterized. A non-synonymous SNP (altering the amino acid sequence from Y to C at position 218) differentiated the tall and dwarf plants and we confirmed, through heterologous yeast transformation, that the IAA uptake capacities of Rc5NG4-1Y and Rc5NG4-1C were significantly different. This study provides insights into the physiological and molecular mechanisms of dwarfing in woody non-timber economically important plants, with potential to aid in the genetic breeding of castor bean and other related crops.
关键词	AUXIN TRANSPORT CELL-WALL ENDOPLASMIC-RETICULUM PLANT HEIGHT ARABIDOPSIS HOMEOSTASIS PROTEIN BIOSYNTHESIS ENCODES SYSTEM
DOI	10.1038/s41598-021-85644-1
WOS记录号	WOS:000667589500013
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73479
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650204, Yunnan, Peoples R China 2.Southwest Forestry Univ, Key Lab Forest Resources Conservat & Utilizat Sou, Minist Educ, Kunming 650224, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang,Zaiqing,Yu,Anmin,Li,Fei,et al. Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean[J]. SCIENTIFIC REPORTS,2021,11(1):6277.
APA	Wang,Zaiqing.,Yu,Anmin.,Li,Fei.,Xu,Wei.,Han,Bing.,...&Liu,Aizhong.(2021).Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean.SCIENTIFIC REPORTS,11(1),6277.
MLA	Wang,Zaiqing,et al."Bulked segregant analysis reveals candidate genes responsible for dwarf formation in woody oilseed crop castor bean".SCIENTIFIC REPORTS 11.1(2021):6277.