Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale

doi:10.3390/plants10040633

KIB OpenIR

	Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale
	Adejobi,Oluwaniyi Isaiah ; Guan,Ju ; Yang,Liu ; Hu,Jiang-Miao ; Yu,Anmin ; Muraguri,Sammy ; Liu,Aizhong
	2021
发表期刊	PLANTS-BASEL
ISSN	2223-7747
卷号	10 期号:4 页码:633
摘要	The Dendrobium plants (members of the Orchidaceae family) are used as traditional Chinese medicinal herbs. Bibenzyl, one of the active compounds in Dendrobium officinale, occurs in low amounts among different tissues. However, market demands require a higher content of thes compounds to meet the threshold for drug production. There is, therefore, an immediate need to dissect the physiological and molecular mechanisms underlying how bibenzyl compounds are biosynthesized in D. officinale tissues. In this study, the accumulation of erianin and gigantol in tissues were studied as representative compounds of bibenzyl. Exogenous application of Methyl-Jasmonate (MeJA) promotes the biosynthesis of bibenzyl compounds; therefore, transcriptomic analyses were conducted between D. officinale-treated root tissues and a control. Our results show that the root tissues contained the highest content of bibenzyl (erianin and gigantol). We identified 1342 differentially expressed genes (DEGs) with 912 up-regulated and 430 down-regulated genes in our transcriptome dataset. Most of the identified DEGs are functionally involved in the JA signaling pathway and the biosynthesis of secondary metabolites. We also identified two candidate cytochrome P450 genes and nine other enzymatic genes functionally involved in bibenzyl biosynthesis. Our study provides insights on the identification of critical genes associated with bibenzyl biosynthesis and accumulation in Dendrobium plants, paving the way for future research on dissecting the physiological and molecular mechanisms of bibenzyl synthesis in plants as well as guide genetic engineering for the improvement of Dendrobium varieties through increasing bibenzyl content for drug production and industrialization.
关键词	bibenzyl Dendrobium officinale transcriptome erianin gigantol FERULATE 5-HYDROXYLASE SECONDARY METABOLITES LIGNIN BIOSYNTHESIS METHYL JASMONATE ANALYSIS REVEALS ACCUMULATION IDENTIFICATION DERIVATIVES EXPRESSION ALKALOIDS
DOI	10.3390/plants10040633
WOS记录号	WOS:000643527700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73477
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Skin Care Prod Codev Ctr Dr Plant, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Southwest Forestry Univ, Minist Educ, Key Lab Forest Resources Conservat & Utilizat Sou, Kunming 650224, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Adejobi,Oluwaniyi Isaiah,Guan,Ju,Yang,Liu,et al. Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale[J]. PLANTS-BASEL,2021,10(4):633.
APA	Adejobi,Oluwaniyi Isaiah.,Guan,Ju.,Yang,Liu.,Hu,Jiang-Miao.,Yu,Anmin.,...&Liu,Aizhong.(2021).Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale.PLANTS-BASEL,10(4),633.
MLA	Adejobi,Oluwaniyi Isaiah,et al."Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale".PLANTS-BASEL 10.4(2021):633.