Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat

doi:10.1021/acs.jafc.0c04277

KIB OpenIR

	Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat
	Han,Chuanyu ; Yu,Zhiyin ; Zhang,Yuting ; Wang,Zhiyan ; Zhao,Junwei ; Huang,Sheng-Xiong ; Ma,Zhonghua ; Wen,Ziyue ; Liu,Chongxi ; Xiang,Wensheng
	2021
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	69 期号:7 页码:2108-2117
摘要	In this study, the supernatant extract from fermentation broth of Streptomyces sp. NEAU-H3 showed strong antifungal activity against Fusarium graminearum strain PH-1 in vitro and in vivo. Three known pyranonaphthoquinones were isolated by means of an activity-guided method, and frenolicin B was characterized as the main active ingredient. Frenolicin B displayed strong antifungal activity against F. graminearum strain PH-1 with an EC50 value of 0.51 mg/L, which is lower than that of carbendazim (0.78 mg/L) but higher than that of phenamacril (0.18 mg/L). Frenolicin B could also strongly inhibit the mycelial growth of Fusarium species, including F. graminearum and F. asiaticum, as well as carbendazim-resistant Fusarium strains isolated from field, with EC50 values of 0.25-0.92 mg/L. Results from field experiments showed that the efficacy of frenolicin B in controlling Fusarium head blight at a treatment concentration of 75 g ai/ha was better than those of phenamacril (375 g ai/ha) and carbendazim (600 g ai/ha) or had no significant difference with that of phenamacril (375 g ai/ha) in 2 years. Scanning electron microscope and transmission electron microscope observations revealed that after treating F. graminearum mycelia with frenolicin B, the mycelia appeared aberrant and had an uneven thickness and swelling, the cytoplasm had disintegrated, and some cell contents were lost. Transcriptome analysis suggests that frenolicin B might inhibit the metabolism of nucleotides and energy by affecting genes involved in phosphorus utilization but did not affect the expression of myosin 5, which is the specific target of phenamacril. These findings indicate that frenolicin B may be a potential agrochemical fungicide for controlling Fusarium head blight.
关键词	frenolicin B Fusarium head blight Fusarium graminearum antifungal activity transcriptome
DOI	10.1021/acs.jafc.0c04277
WOS记录号	WOS:000624326000009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73474
专题	中国科学院昆明植物研究所
作者单位	1.Northeast Agr Univ, Key Lab Agr Microbiol Heilongjiang Prov, Harbin 150030, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China 4.Zhejiang Univ, Inst Biotechnol, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China 5.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Han,Chuanyu,Yu,Zhiyin,Zhang,Yuting,et al. Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2021,69(7):2108-2117.
APA	Han,Chuanyu.,Yu,Zhiyin.,Zhang,Yuting.,Wang,Zhiyan.,Zhao,Junwei.,...&Xiang,Wensheng.(2021).Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(7),2108-2117.
MLA	Han,Chuanyu,et al."Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.7(2021):2108-2117.