Regulation of the Development in Physcomitrium (Physcomitrella) patens implicates the functional differentiation of plant RNase H1s

doi:10.1016/j.plantsci.2021.111070

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	Regulation of the Development in Physcomitrium (Physcomitrella) patens implicates the functional differentiation of plant RNase H1s
	Chen,Silin ; Dong,Xiumei ; Yang,Zhuo ; Hou,Xin ; Liu,Li
	2021
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	313 页码:111070
摘要	R-loops, consisting of a DNA:RNA hybrid and a single-stranded DNA (ssDNA), form naturally as functional chromosome structures and are crucial in many vital biological processes. However, disrupted R-loop homeo-stasis will threat to the integrity and stability of genome. As the endonuclease, RNase H1 can efficiently recognize and remove excess R-loops to protect organisms from DNA damage induced by R-loop over-accumulation. Here, we investigated the function of RNase H1 in Physcomitrium (Physcomitrella) patens to illustrate its important role in the evolution of plants. We found that PpRNH1A dysfunction seriously affected shoot growth and branch formation in P. patens, revealing a noticeable functional difference between PpRNH1A and AtRNH1A of Arabi-dopsis. Furthermore, auxin signaling was significantly affected at the transcriptional level in PpRNH1A mutant plants, as a result of the accumulation of R-loops at several auxin-related genes. This study provides evidence that PpRNH1A regulates the development of P. patens by controlling R-loop formation at specific loci to modulate the transcription of auxin-related genes. It also highlights the interspecific functional differences between early land plants and vascular plants, despite crucial and conserved role of RNase H1 played in maintaining R-loop homeostasis.
关键词	Physcomitrium patens Plant development RNase H1 Auxin R-loop R-LOOP FORMATION GC SKEW MITOCHONDRIAL
DOI	10.1016/j.plantsci.2021.111070
WOS记录号	WOS:000709990700006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73464
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Ke, Wuhan 430062, Peoples R China 4.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Chen,Silin,Dong,Xiumei,Yang,Zhuo,et al. Regulation of the Development in Physcomitrium (Physcomitrella) patens implicates the functional differentiation of plant RNase H1s[J]. PLANT SCIENCE,2021,313:111070.
APA	Chen,Silin,Dong,Xiumei,Yang,Zhuo,Hou,Xin,&Liu,Li.(2021).Regulation of the Development in Physcomitrium (Physcomitrella) patens implicates the functional differentiation of plant RNase H1s.PLANT SCIENCE,313,111070.
MLA	Chen,Silin,et al."Regulation of the Development in Physcomitrium (Physcomitrella) patens implicates the functional differentiation of plant RNase H1s".PLANT SCIENCE 313(2021):111070.