Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates

doi:10.3389/fmicb.2021.636344

KIB OpenIR

	Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates
	Cai,YingLi ; Ma,XiaoLong ; Zhang,QianQian ; Yu,FuQiang ; Zhao,Qi; Huang,Wei; Song,JiaXin ; Liu,Wei
	2021
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	12 页码:636344
摘要	Morels (Morchella sp.) are economically important edible macro-fungi, which can grow on various synthetic or semi-synthetic media. However, the complex nutritional metabolism and requirements of these fungi remain ill-defined. This study, based on the plant biomass commonly used in the artificial cultivation of morels, assessed and compared the growth characteristics and extracellular enzymes of Morchella importuna cultivated on glucose, rice straw, sawdust, wheat grain, and a mixture of equal proportions of the three latter plant substrates (MIX). M. importuna could grow on all five tested media but displayed significant variations in mycelial growth rate, biomass, and sclerotium yield on the different media. The most suitable medium for M. importuna was wheat and wheat-containing medium, followed by glucose, while rice straw and sawdust were the least suitable. A total of 268 secretory proteins were identified by liquid chromatography coupled with tandem mass spectrometry detection. Functional classification and label-free comparative analysis of these proteins revealed that carbohydrate-active enzyme (CAZYme) proteins were the predominant component of the secretome of M. importuna, followed by protease, peptidase, and other proteins. The abundances of CAZYme proteins differed among the tested media, ranging from 64% on glucose to 88% on rice straw. The CAZYme classes of glycoside hydrolases and carbohydrate-binding module were enriched in the five secretomes. Furthermore, the enzyme activities of CMCase, lignase, amylase, xylase, pNPCase, and pNPGase were detected during the continuous culture of M. importuna in MIX medium, and the relative expression of the corresponding genes were detected by quantitative real-time PCR. The combined data of growth potential, secretome, extracellular enzyme activity, and gene expression on different substrates inferred that M. importuna was weak in lignocellulose degradation but a good starch decomposer. Specifically, in terms of the degradation of cellulose, the ability to degrade cellulose into oligosaccharides was weaker compared with further degradation into monosaccharides, and this might be the speed-limiting step of cellulose utilization in M. importuna. In addition, M. importuna had a strong ability to decompose various hemicellulose glycosidic bonds, especially alpha- and beta-galactosidase. Only a very few lignin-degradation-related proteins were detected, and these were in low abundance, consistent with the presence of weak lignin degradation ability. Furthermore, the presence of lipase and chitinase implied that M. importuna was capable of decomposition of its own mycelia in vitro. The study provides key data that facilitates a further understanding of the complex nutritional metabolism of M. importuna.
关键词	secretory proteome enzyme activity substrate utilization amylase nutritional metabolism CAZYme LIGNINOLYTIC ENZYME-PRODUCTION CELLULOSE DEGRADATION BROWN-ROT FUNGAL WOOD MECHANISMS ROOTS IDENTIFICATION ASSOCIATION INSIGHT
DOI	10.3389/fmicb.2021.636344
WOS记录号	WOS:000658494900001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73453
专题	中国科学院昆明植物研究所
作者单位	1.Wuhan Acad Agr Sci, Inst Vegetable, Wuhan, Peoples R China 2.Huazhong Agr Univ, Inst Appl Mycol, Wuhan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Germplasm Bank Wild Species Southwestern China, Kunming, Yunnan, Peoples R China 4.Southwest Forestry Univ, Inst Appl Mycol, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Cai,YingLi,Ma,XiaoLong,Zhang,QianQian,et al. Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates[J]. FRONTIERS IN MICROBIOLOGY,2021,12:636344.
APA	Cai,YingLi.,Ma,XiaoLong.,Zhang,QianQian.,Yu,FuQiang.,Zhao,Qi.,...&Liu,Wei.(2021).Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates.FRONTIERS IN MICROBIOLOGY,12,636344.
MLA	Cai,YingLi,et al."Physiological Characteristics and Comparative Secretome Analysis of Morchella importuna Grown on Glucose, Rice Straw, Sawdust, Wheat Grain, and MIX Substrates".FRONTIERS IN MICROBIOLOGY 12(2021):636344.