Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach

doi:10.3390/jof7030204

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	Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach
	Zhou,Shengwen ; Li,Xincan ; Luli,Yunjiao ; Li,Xuan ; Chen,Zuo H.; Yuan,Pengcheng ; Yang,Zhu L.; Li,Guohong ; Luo,Hong
	2021
发表期刊	JOURNAL OF FUNGI
ISSN	2309-608X
卷号	7 期号:3 页码:204
摘要	Most species in the genus Amanita are ectomycorrhizal fungi comprising both edible and poisonous mushrooms. Some species produce potent cyclic peptide toxins, such as alpha-amanitin, which places them among the deadliest organisms known to mankind. These toxins and related cyclic peptides are encoded by genes of the MSDIN family (named after the first five amino acid residues of the precursor peptides), and it is largely unknown to what extent these genes are expressed in the basidiocarps. In the present study, Amanita rimosa and Amanita exitialis were sequenced through the PacBio and Illumina techniques. Together with our two previously sequenced genomes, Amanita subjunquillea and Amanita pallidorosea, in total, 46 previously unknown MSDIN genes were discovered. The expression of over 80% of the MSDIN genes was demonstrated in A. subjunquillea. Through a combination of genomics and mass spectrometry, 12 MSDIN genes were shown to produce novel cyclic peptides. To further confirm the results, three of the cyclic peptides were chemically synthesized. The tandem mass spectrometry (MS/MS) spectra of the natural and the synthetic peptides shared a majority of the fragment ions, demonstrating an identical structure between each peptide pair. Collectively, the results suggested that the genome-guided approach is reliable for identifying novel cyclic peptides in Amanita species and that there is a large peptide reservoir in these mushrooms.
关键词	poisonous mushroom genome MSDIN family Amanitin LC– HRMS
DOI	10.3390/jof7030204
WOS记录号	WOS:000633768200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73433
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Div & Green Dev, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Kunming Univ Sci & Technol, Dept Environm Sci & Engn, Kunming 650091, Yunnan, Peoples R China 6.Chen, Zuo H.] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zhou,Shengwen,Li,Xincan,Luli,Yunjiao,et al. Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach[J]. JOURNAL OF FUNGI,2021,7(3):204.
APA	Zhou,Shengwen.,Li,Xincan.,Luli,Yunjiao.,Li,Xuan.,Chen,Zuo H..,...&Luo,Hong.(2021).Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach.JOURNAL OF FUNGI,7(3),204.
MLA	Zhou,Shengwen,et al."Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach".JOURNAL OF FUNGI 7.3(2021):204.

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