Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides in Amanita molliuscula

doi:10.3390/jof7050384

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	Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides in Amanita molliuscula
	Luli,Yunjiao ; Zhou,Shengwen ; Li,Xuan ; Chen,Zuohong ; Yang,Zhuliang; Luo,Hong
	2021
发表期刊	JOURNAL OF FUNGI
ISSN	2309-608X
卷号	7 期号:5 页码:384
摘要	Amanita molliuscula is a basal species of lethal Amanita and intrigues the field because it does not produce discernable alpha-amanitin when inspected by High Performance Liquid Chromatography (HPLC), which sets it apart from all known amanitin-producing (lethal) Amanita species. In order to study the underlining genetic basis of the phenotype, we sequenced this species through PacBio and Illumina RNA-Seq platforms. In total, 17 genes of the MSDIN family (named after the first five amino acid residues of the precursor peptides) were found in the genome and 11 of them were expressed at the transcription level. The expression pattern was not even but in a differential fashion: two of the MSDINs were highly expressed (FPKM value > 100), while the majority were expressed at low levels (FPKM value < 1). Prolyl oligopeptidease B (POPB) is the key enzyme in the amanitin biosynthetic pathway, and high expression of this enzyme was also discovered (FPKM value > 100). The two MSDINs with highest transcription further translated into two novel cyclic peptides, the structure of which is distinctive from all known cyclic peptides. The result illustrates the correlation between the expression and the final peptide products. In contrast to previous HPLC result, the genome of A. molliuscula harbors alpha-amanitin genes (three copies), but the product was in trace amount indicated by MS. Overall, transcription of MSDINs encoding major toxins (alpha-amanitin, beta-amanitin, phallacidin and phalloidin) were low, showing that these toxins were not actively synthesized at the stage. Collectively, our results indicate that the amanitin biosynthetic pathway is highly active at the mature fruiting body stage in A. molliuscula, and due to the differential expression of MSDIN genes, the pathway produces only a few cyclic peptides at the time.
关键词	poisonous mushroom genome transcriptome MSDIN family MS MUSHROOM POISONING OUTBREAKS ALPHA-AMANITIN GENOME FAMILY TRANSCRIPTOME PHALLOIDES PHYLOGENY DIVERSITY INFERENCE TAXONOMY
DOI	10.3390/jof7050384
WOS记录号	WOS:000654199200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73432
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 5.Kunming Univ Sci & Technol, Dept Environm Sci & Engn, Kunming 650091, Yunnan, Peoples R China 6.Hunan Normal Univ, Coll Life Sci, Changsha 410081, Peoples R China
推荐引用方式 GB/T 7714	Luli,Yunjiao,Zhou,Shengwen,Li,Xuan,et al. Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides in Amanita molliuscula[J]. JOURNAL OF FUNGI,2021,7(5):384.
APA	Luli,Yunjiao,Zhou,Shengwen,Li,Xuan,Chen,Zuohong,Yang,Zhuliang,&Luo,Hong.(2021).Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides in Amanita molliuscula.JOURNAL OF FUNGI,7(5),384.
MLA	Luli,Yunjiao,et al."Differential Expression of Amanitin Biosynthetic Genes and Novel Cyclic Peptides in Amanita molliuscula".JOURNAL OF FUNGI 7.5(2021):384.

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