Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo

doi:10.1016/j.jep.2020.113506

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	Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo
	Zhao,Yun-Li ; Pu,Shi-Biao ; Qi,Yan ; Wu,Bai-Fen ; Shang,Jian-Hua ; Liu,Ya-Ping ; Hu,Di ; Luo,Xiao-Dong
	2021
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	267 页码:113506
摘要	Ethnopharmacological relevance: Alstonia scholaris (L.) R. Br. (Apocynaceae) is a Dai folk medicine for the treatment of lung diseases in China.Aim of the study: The present study investigated the anti-pulmonary fibrosis effects of total alkaloids (TA) and the potential active ingredients and its possible mechanism.Materials and methods: After intratracheal instillation of bleomycin (BLM, 5 mg/kg), mice were divided into ten groups, and orally treated with the corresponding samples once daily for 28 days. The effect of indole alkaloids was determined through analysis of cytokines, as well as histopathological examinations and gene expressions.Results: Severe lung fibrosis was observed in the BLM-treated mice on day 28. However, the administration of TA significantly ameliorated the pathological changes in the lungs, decreased the content of Krebs von den Lungen-6, lactate dehydrogenase, transforming growth factor-beta (TGF-beta), hydroxyproline, type I collagen, and malonaldehyde, and enhanced the activity of superoxide dismutase in the serum and lung tissues. In addition, the enhanced TGF-beta and matrix metalloproteinase-1 (MMP-1) expressions in BLM-induced mice were obviously weakened by indole alkaloids, as well as the ratio of matrix metalloproteinase-1 to tissue inhibitor of metalloproteinase-1 was decreased. Moreover, picrinine and scholaricine yielded markedly better values in the aforementioned indices than those in other samples, indicating that they may be the active ingredients of alkaloids.Conclusions: TA exerted protective effects against BLM-induced pulmonary fibrosis by reducing collagen deposition through TGF-beta/MMP-1 pathway.
关键词	Alstonia scholaris Indole alkaloids Pulmonary fibrosis Picrinine Vallesamine Scholaricine 19-Epischolaricine FLIGHT MASS-SPECTROMETRY ACUTE LUNG INJURY AIRWAY INFLAMMATION OXIDATIVE STRESS BARK EXTRACTS LEAVES CONSTITUENTS COLLAGEN LEAF KL-6
DOI	10.1016/j.jep.2020.113506
WOS记录号	WOS:000606365200004
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73426
专题	中国科学院昆明植物研究所
作者单位	1.Yunnan Univ, Key Lab Med Chem Nat Resource, Minist Educ & Yunnan Prov, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Univ Chinese Med, Chinese Mat Med, Kunming 650500, Yunnan, Peoples R China 4.Yunnan Prov Hosp TCM, Kunming 650021, Yunnan, Peoples R China 5.Yunnan Univ Business Management, Kunming 650500, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Zhao,Yun-Li,Pu,Shi-Biao,Qi,Yan,et al. Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo[J]. JOURNAL OF ETHNOPHARMACOLOGY,2021,267:113506.
APA	Zhao,Yun-Li.,Pu,Shi-Biao.,Qi,Yan.,Wu,Bai-Fen.,Shang,Jian-Hua.,...&Luo,Xiao-Dong.(2021).Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo.JOURNAL OF ETHNOPHARMACOLOGY,267,113506.
MLA	Zhao,Yun-Li,et al."Pharmacological effects of indole alkaloids from Alstonia scholaris (L.) R. Br. on pulmonary fibrosis in vivo".JOURNAL OF ETHNOPHARMACOLOGY 267(2021):113506.

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