Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata

doi:10.1016/j.pld.2020.11.0032468-2659

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	Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata
	Ma,Lan ; Li,Rongping ; Ma,Luoyan ; Song,Na ; Xu,Zhen ; Wu,Jinsong
	2021
发表期刊	PLANT DIVERSITY
ISSN	2096-2703
卷号	43 期号:6 页码:502-509
摘要	NAC-LIKE, ACTIVATED BY AP3/PI (NAP) is a NAC transcription factor regulating leaf senescence in Arabidopsis thaliana. In wild tobacco Nicotiana attenuata, a nuclear localized NAC transcription factor NaNAC29 was identified to be highly elicited after inoculation of Alternaria alternata, a notorious necrotic fungus on tobacco species. The NaNAC29 possesses similar tertiary structure to NAP with 60% amino acid identity. However, it remains unknown the role of NaNAC29 in plant defense responses to A. alternata and leaf senescence in N. attenuata. In this paper, Defensin-like protein 1 (NaDLP1) was highly induced in N. attenuata after A. alternata inoculation and bigger lesions were developed in NaDLP1-silenced plants. Interestingly, A. alternata-induced NaDLP1 was reduced by 76% in VIGS NaNAC29 plants and by 61% in JA deficient irAOC plants at 3 days post inoculation. The regulation of NaDLP1 expression by NaNAC29 was clearly independent on JA pathway, since exogenous methyl jasmonate treatment could not complement the induction levels of NaDLP1 in NaNAC29-silenced plants to the levels in WT plants. Otherwise, the expression of NaNAC29 was low expressed in young leaves but highly in senescent leaves and dark treated leaves. NaNAC29-silenced plants, which were generated by virus-induced gene silencing (VIGS NaNAC29), showed delayed senescence phenotype. In addition, constitutive over-expression of NaNAC29 in A. thaliana could rescue the delayed-senescence phenotype of nap and caused precocious leaf senescence of wild-type Col-0 plants. All the data above demonstrate that NaNAC29 is a NAP homolog in N. attenuata participating in the defense responses to A. alternata by regulation of a defensin protein NaDLP1 and promoting leaf senescence. Copyright (c) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
关键词	NaNAC29 Defensin-like protein 1 Tobacco Brown spot disease Aging ABA BIOSYNTHESIS GENE ACID PHYTOALEXIN EXPRESSION SCOPOLETIN DEFENSIN ORA59 ATNAP
DOI	10.1016/j.pld.2020.11.0032468-2659
WOS记录号	WOS:000735917200001
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73413
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Lanhei Rd 132, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma,Lan,Li,Rongping,Ma,Luoyan,et al. Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata[J]. PLANT DIVERSITY,2021,43(6):502-509.
APA	Ma,Lan,Li,Rongping,Ma,Luoyan,Song,Na,Xu,Zhen,&Wu,Jinsong.(2021).Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata.PLANT DIVERSITY,43(6),502-509.
MLA	Ma,Lan,et al."Involvement of NAC transcription factor NaNAC29 in Alternaria alternata resistance and leaf senescence in Nicotiana attenuata".PLANT DIVERSITY 43.6(2021):502-509.