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Large-Scale Characterization of the Soil Microbiome in Ancient Tea Plantations Using High-Throughput 16S rRNA and Internal Transcribed Spacer Amplicon Sequencing
Kui,Ling; Xiang,Guisheng; Wang,Ya; Wang,Zijun; Li,Guorong; Li,Dawei; Yan,Jing; Ye,Shuang; Wang,Chunping; Yang,Ling; Zhang,Shiyu; Zhang,Shuangyan; Zhou,Ling; Gui,Heng; Xu,Jianchu; Chen,Wei; Zhang,Jun; Huang,Tingyuan; Majeed,Aasim; Sheng,Jun; Dong,Yang
2021
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume12Pages:745225
AbstractThere is a special interaction between the environment, soil microorganisms, and tea plants, which constitute the ecosystem of tea plantations. Influenced by environmental factors and human management, the changes in soil microbial community affected the growth, quality, and yield of tea plants. However, little is known about the composition and structure of soil bacterial and fungal communities in 100-year-old tea plantations and the mechanisms by which they are affected. In this regard, we characterized the microbiome of tea plantation soils by considering the bacterial and fungal communities in 448 soil samples from 101 ancient tea plantations in eight counties of Lincang city, which is one of the tea domestication centers in the world. 16S and Internal Transcribed Spacer (ITS) rRNA high-throughput amplicon sequencing techniques were applied in this study. The results showed that the abundance, diversity, and composition of the bacterial and fungal communities have different sensitivity with varying pH, altitude, and latitude. pH and altitude affect soil microbial communities, and bacterial communities are more sensitive than fungi in terms of abundance and diversity to pH. The highest alpha-diversity of bacterial communities is shown in the pH 4.50-5.00 and 2,200-m group, and fungi peaked in the pH 5.00-5.50 and 900-m group. Because of environmental and geographical factors, all microbes are similarly changing, and further correlations showed that the composition and structure of bacterial communities are more sensitive than fungal communities, which were affected by latitude and altitude. In conclusion, the interference of anthropogenic activities plays a more important role in governing fungal community selection than environmental or geographical factors, whereas for the bacterial community, it is more selective to environment adaptation than to adaptation to human activities.
KeywordCamellia sinensis var assamica microbiome function ancient tea plantations 16S and ITS rRNA FUNGAL COMMUNITIES CAMELLIA-SINENSIS BACTERIAL COMMUNITIES ALTITUDINAL GRADIENT ORCHARD SOILS ACIDIFICATION RHIZOSPHERE DIVERSITY GROWTH NITROGEN
DOI10.3389/fmicb.2021.745225
WOS IDWOS:000715223800001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/73406
Collection中国科学院昆明植物研究所
Affiliation1.Shenzhen Qianhai Shekou Free Trade Zone Hosp, Shenzhen, Peoples R China
2.Yunnan Agr Univ, Appl Genom Technol Lab, Sch Biol Big Data, Kunming, Yunnan, Peoples R China
3.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming, Yunnan, Peoples R China
4.Lincang Tea Res Inst, Lincang, Peoples R China
5.Yunnan Agr Univ, Longrun Puer Tea Coll, Kunming, Yunnan, Peoples R China
6.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
7.Chinese Acad Sci, Ctr Mt Futures, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
8.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming, Yunnan, Peoples R China
9.Guangzhou Ctr Dis Control & Prevent, Guangzhou, Peoples R China
10.Cent Univ Punjab, Mol Genet Lab, Lahore, India
11.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China
12.Yunnan Res Inst Local Plateau Agr & Ind, Kunming, Yunnan, Peoples R China
13.Yunnan Agr Univ, Key Lab Agrobiodivers & Pest Control, Minist Educ, Kunming, Yunnan, Peoples R China
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GB/T 7714
Kui,Ling,Xiang,Guisheng,Wang,Ya,et al. Large-Scale Characterization of the Soil Microbiome in Ancient Tea Plantations Using High-Throughput 16S rRNA and Internal Transcribed Spacer Amplicon Sequencing[J]. FRONTIERS IN MICROBIOLOGY,2021,12:745225.
APA Kui,Ling.,Xiang,Guisheng.,Wang,Ya.,Wang,Zijun.,Li,Guorong.,...&Dong,Yang.(2021).Large-Scale Characterization of the Soil Microbiome in Ancient Tea Plantations Using High-Throughput 16S rRNA and Internal Transcribed Spacer Amplicon Sequencing.FRONTIERS IN MICROBIOLOGY,12,745225.
MLA Kui,Ling,et al."Large-Scale Characterization of the Soil Microbiome in Ancient Tea Plantations Using High-Throughput 16S rRNA and Internal Transcribed Spacer Amplicon Sequencing".FRONTIERS IN MICROBIOLOGY 12(2021):745225.
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