De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases

doi:10.1016/j.abb.2020.108742

KIB OpenIR

	De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases
	Yan,Xiaoguang ; Li,Weiguo ; Liang,Dongmei ; Caiyin,Qinggele ; Zhao,Guangrong ; Zhang,Zhijun ; Ma Wenzhang7 ; Qiao,Jianjun
	2021
发表期刊	ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN	0003-9861
卷号	698 页码:108742
摘要	Mylia taylorii is an ancient nonseed land plant that accumulates various sesquiterpenes with insecticidal and antibacterial activities. Recently, microbial-type sesquiterpene synthases (STSs) with atypical aspartate-rich metal ion binding motifs have been identified in some liverworts. Here, transcriptome analysis of M. taylorii was performed to identify M. taylorii sesquiterpene synthases (MtSTSs) that are potentially involved in sesquiterpene biosynthesis and diversity. A total of 255,669 unigenes were obtained with an average length of 963 bp in the transcriptome data of M. taylorii, among which 148,093 (57.92%) unigenes had BLAST results. Forty-eight unigenes were related to the sesquiterpene backbone biosynthesis according to KEGG annotation. In addition, MtSTS1, MtSTS2 and MtSTS3 identified from putative MtSTSs display sesquiterpene catalytic activities on the basis of functional characterizations in yeast. Interestingly, MtSTSs exhibit a noncanonical metal ion binding motif and the structural composition of a single alpha-domain, which are features of microbial STSs instead of archetypical plant STSs. This study revealed new microbial-type STS members of nonseed plants, and functionally identified that MtSTSs may contribute to the investigation of the biosynthesis and biological role of sesquiterpenes in M. taylorii.
关键词	Liverwort Microbial-type sesquiterpene synthase Mylia taylorii Transcriptome analysis TERPENE SYNTHASES ARISTOLOCHENE SYNTHASE MARCHANTIA-POLYMORPHA CRYSTAL-STRUCTURE CONSTITUENTS BIOSYNTHESIS NEROLIDOL DIVERSITY EVOLUTION PLANTS
DOI	10.1016/j.abb.2020.108742
WOS记录号	WOS:000609479600001
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73353
专题	中国科学院昆明植物研究所
作者单位	1.Tianjin Univ, Sch Chem Engn & Technol, Dept Pharmaceut Engn, Tianjin 300072, Peoples R China 2.Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China 3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, SynBio Res Platform, Tianjin 300072, Peoples R China 4.Tianjin Res Inst Forestry & Pomol, Tianjin 300384, Peoples R China 5.Natl Engn Technol Res Ctr Preservat Agr Prod, Tianjin 300384, Peoples R China 6.Minist Agr & Rural Affairs, Key Lab Storage Agr Prod, Tianjin 300384, Peoples R China 7.Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China 8.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yan,Xiaoguang,Li,Weiguo,Liang,Dongmei,et al. De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2021,698:108742.
APA	Yan,Xiaoguang.,Li,Weiguo.,Liang,Dongmei.,Caiyin,Qinggele.,Zhao,Guangrong.,...&Qiao,Jianjun.(2021).De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,698,108742.
MLA	Yan,Xiaoguang,et al."De novo assembly of the Mylia taylorii transcriptome and identification of sesquiterpene synthases".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 698(2021):108742.