Inositol polyphosphate multikinase IPMK-1 regulates development through IP3/calcium signaling in Caenorhabditis elegans

doi:10.1016/j.ceca.2020.102327

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	Inositol polyphosphate multikinase IPMK-1 regulates development through IP3/calcium signaling in Caenorhabditis elegans
	Yang,Zhong-Lin ; Chen,Jian-Ning ; Lu,Yu-Yang ; Lu,Min ; Wan,Qin-Li ; Wu,Gui-Sheng ; Luo,Huai-Rong
	2021
发表期刊	CELL CALCIUM
ISSN	0143-4160
卷号	93 页码:102327
摘要	Inositol polyphosphate multikinase (IPMK) is a conserved protein that initiates the production of inositol phosphate intracellular messengers and is critical for regulating a variety of cellular processes. Here, we report that the C. elegans IPMK-1, which is homologous to the mammalian inositol polyphosphate multikinase, plays a crucial role in regulating rhythmic behavior and development. The deletion mutant ipmk-1(tm2687) displays a long defecation cycle period and retarded postembryonic growth. The expression of functional ipmk-1::GFP was detected in the pharyngeal muscles, amphid sheath cells, the intestine, excretory (canal) cells, proximal gonad, and spermatheca. The expression of IPMK-1 in the intestine was sufficient for the wild-type phenotype. The IP3-kinase activity of IPMK-1 is required for defecation rhythms and postembryonic development. The defective phenotypes of ipmk-1(tin2687) could be rescued by a loss-of-function mutation in type I inositol 5-phosphatase homolog (IPP-5) and improved by a supplemental Ca2+ in the medium. Our work demonstrates that IPMK-1 and the signaling molecule inositol triphosphate (IP3) pathway modulate rhythmic behaviors and development by dynamically regulating the concentration of intracellular Ca2+ in C. elegans. Advances in understanding the molecular regulation of Ca2+ homeostasis and regulation of organism development may lead to therapeutic strategies that modulate Ca2+ signaling to enhance function and counteract disease processes. Unraveling the physiological role of IPMK and the underlying functional mechanism in C. elegans would contribute to understanding the role of IPMK in other species, especially in mammals, and benefit further research on the involvement of IPMK in disease.
关键词	Inositol polyphosphates Inositol polyphosphate multikinase Type I inositol 5-phosphatase Development Calcium signaling Defecation rhythm C. elegans C-ELEGANS PHOSPHOLIPASE-C CALCIUM 1,4,5-TRISPHOSPHATE CALCINEURIN KINASE IDENTIFICATION TRISPHOSPHATE METABOLISM RECEPTOR
DOI	10.1016/j.ceca.2020.102327
WOS记录号	WOS:000607496300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73214
专题	中国科学院昆明植物研究所
作者单位	1.[Yang, Zhong-Lin 2.Wu, Gui-Sheng 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.[Chen, Jian-Ning 6.Lu, Yu-Yang 7.Lu, Min 8.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Key Lab Aging & Regenerat Med, 319 Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China 9.Jinan Univ, Ctr Precis Med, Biomed Translat Res Inst, Affiliated Hosp 1, Guangzhou 510632, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Yang,Zhong-Lin,Chen,Jian-Ning,Lu,Yu-Yang,et al. Inositol polyphosphate multikinase IPMK-1 regulates development through IP3/calcium signaling in Caenorhabditis elegans[J]. CELL CALCIUM,2021,93:102327.
APA	Yang,Zhong-Lin.,Chen,Jian-Ning.,Lu,Yu-Yang.,Lu,Min.,Wan,Qin-Li.,...&Luo,Huai-Rong.(2021).Inositol polyphosphate multikinase IPMK-1 regulates development through IP3/calcium signaling in Caenorhabditis elegans.CELL CALCIUM,93,102327.
MLA	Yang,Zhong-Lin,et al."Inositol polyphosphate multikinase IPMK-1 regulates development through IP3/calcium signaling in Caenorhabditis elegans".CELL CALCIUM 93(2021):102327.

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