NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata

doi:10.1007/s00299-020-02616-x

KIB OpenIR

	NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata
	Yin,Min ; Song,Na ; Chen,Suiyun ; Wu,Jinsong
	2021
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	40 期号:1 页码:97-109
摘要	Key message Here, we reported that a pathogen- and herbivore-induced Kunitz trypsin inhibitor gene, NaKTI2, is required for herbivore resistance, and transcriptionally regulated mainly by NaWRKY3 and NaWRKY6 but not Jasmonate signaling. Plant protease inhibitor (PI) occurs widely in plant species, and is considered as an important part of plant defense arsenal against herbivores. Transcriptome analysis ofNicotiana attenuataleaves revealed that a Kunitz trypsin inhibitor gene,NaKTI2, was highly elicited after inoculation ofAlternaria alternata(tobacco pathotype). However, the roles ofNaKTI2in pathogen- and herbivore resistance and its regulation were unclear. NaKTI2 had typical domains of Kunitz trypsin inhibitors and exhibited a high level of trypsin protease inhibitor activities when transiently over-expressed. The transcripts ofNaKTI2could be induced byA. alternataandSpodoptera lituraoral secretions (OS). SilencingNaKTI2via virus-induced gene silencing technique has no influence on lesion diameters developed onN. attenuataleaves afterA. alternatainoculation, butS. lituralarvae gained more mass and had higher survivorship onNaKTI2-silenced plants. Meanwhile, the expression ofNaPI, a PI gene essential for herbivore resistance previously identified inN. attenuata, was not affected inNaKTI2-silenced plants. UnlikeNaPI, which was predominantly regulated by jasmonate (JA) signaling, OS-elicitedNaKTI2transcripts were only slightly reduced in JA-deficient plants, but were dramatically decreased inNaWRKY3- andNaWRKY6- silenced plants, respectively. Further electromobility shift assays indicated that NaWRKY3 and NaWRKY6 could directly bind to the promoter regions ofNaKTI2in vitro. Taken together, our results demonstrate that in addition toNaPI,NaKTI2, a pathogen- and herbivore-induced Kunitz trypsin inhibitor gene, is also required for herbivore resistance, and mainly regulated by NaWRKY3 and NaWRKY6.
关键词	Nicotiana attenuata Kunitz trypsin inhibitor Alternaria alternata Spodoptera litura WRKY NICOTIANA-ATTENUATA PROTEINASE-INHIBITOR PHYTOALEXIN SCOPOLETIN ALTERNARIA-ALTERNATA ANTIFUNGAL ACTIVITY DEFENSE RESPONSES PLANT-RESPONSES TOBACCO VIRUS GENE
DOI	10.1007/s00299-020-02616-x
WOS记录号	WOS:000578104000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73207
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Univ, Biocontrol Engn Res Ctr Crop Dis & Pest, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 4.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
推荐引用方式 GB/T 7714	Yin,Min,Song,Na,Chen,Suiyun,et al. NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata[J]. PLANT CELL REPORTS,2021,40(1):97-109.
APA	Yin,Min,Song,Na,Chen,Suiyun,&Wu,Jinsong.(2021).NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata.PLANT CELL REPORTS,40(1),97-109.
MLA	Yin,Min,et al."NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata".PLANT CELL REPORTS 40.1(2021):97-109.