Requirement of Jasmonate signaling for defense responses against Alternaria alternata and Phytophthora nicotiane in tobacco

doi:10.1002/csc2.20625

KIB OpenIR

	Requirement of Jasmonate signaling for defense responses against Alternaria alternata and Phytophthora nicotiane in tobacco
	Long,Jingyu ; Yang,Mao ; Zuo,Cailian ; Song,Na ; He,Jun-Min ; Zeng,Jianmin ; Wu,Jinsong
	2021
发表期刊	CROP SCIENCE
ISSN	0011-183X
卷号	61 期号:6 页码:4273-4283
摘要	Alternaria alternata and Phytophthora nicotianae are two major necrotrophic pathogens of tobacco. It is currently unknown whether they elicit the same suite of defense responses and whether jasmonate (JA) signaling plays an important role in the defense response of cultivated tobacco against these two pathogens. During transcriptome analysis, we found some conserved defense responses in the flue-cured tobacco cultivar K326 against A. alternata and P. nicotianae. Of the top 20 significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways, eight were shared in 'K326' plants inoculated with A. alternata or P. nicotianae; these included the phenylpropanoid biosynthesis and the sesquiterpenoid biosynthesis pathways. Induction of expression of the genes feruloyl-CoA 6 '-hydroxylase 1 (F6'H1), 5-epi-aristolochene synthase (EAS), and 5-epi-aristolochene hydroxylase (EAH) for biosynthesis of the phytoalexins scopoletin (F6'H1) and capsidiol (EAS and EAH) was further confirmed by real-time polymerase chain reaction, and they are representative genes of phenylpropanoid and sesquiterpenoid pathways. Further analysis showed that the levels of JA and JA-Ile, and transcripts of allene oxide cyclase (AOC), the key enzyme gene for JA biosynthesis, were also significantly induced by both pathogens in 'K326.' Plants silenced for either AOC or Coronatine Insensitive 1 (COI1), the gene encoding the JA-Ile receptor, were highly susceptible to infection by both pathogens. Taken together, our results show that defense responses, especially the activation of the pathways for biosynthesis of the phytoalexins scopoletin and capsidiol, are conserved in N. attenuata and cultivated tobacco infected by A. alternata and P. nicotianae, and provided compelling evidence for the role of JA signaling in the defense against these two pathogens.
关键词	PHYTOALEXIN SCOPOLETIN ATTENUATA RESISTANCE ACTIVATION ETHYLENE PATHWAY GENE
DOI	10.1002/csc2.20625
WOS记录号	WOS:000708446100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73206
专题	中国科学院昆明植物研究所
作者单位	1.Shaanxi Normal Univ, Sch Life Sci, Xian 710062, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yunnan Acad Tobacco Agr Sci, Kunming 650021, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Long,Jingyu,Yang,Mao,Zuo,Cailian,et al. Requirement of Jasmonate signaling for defense responses against Alternaria alternata and Phytophthora nicotiane in tobacco[J]. CROP SCIENCE,2021,61(6):4273-4283.
APA	Long,Jingyu.,Yang,Mao.,Zuo,Cailian.,Song,Na.,He,Jun-Min.,...&Wu,Jinsong.(2021).Requirement of Jasmonate signaling for defense responses against Alternaria alternata and Phytophthora nicotiane in tobacco.CROP SCIENCE,61(6),4273-4283.
MLA	Long,Jingyu,et al."Requirement of Jasmonate signaling for defense responses against Alternaria alternata and Phytophthora nicotiane in tobacco".CROP SCIENCE 61.6(2021):4273-4283.