Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions

doi:10.3390/ijms22179599

KIB OpenIR

	Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions
	Yang,Danni ; Ni,Ruize ; Yang,Shihai ; Pu,Yanan ; Qian,Min ; Yang,Yunqiang; Yang,Yongping
	2021
Source Publication	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
Volume	22 Issue:17 Pages:9599
Abstract	Free proline has multiple functions in plant cells, such as regulating osmotic potential and protecting both proteins and cell membranes. The expression of Delta 1-Pyrroline-5-carboxylate synthase (P5CS), a key enzyme in the proline biosynthetic pathway, increases under drought, salt and cold stress conditions, causing plant cells to accumulate large amounts of proline. In this study, we cloned and identified the P5CS gene from Stipa purpurea, which has a full-length of 2196 bp and encodes 731 amino acids. A subcellular localization analysis indicated that SpP5CS localized to the cytoplasm. The ectopic overexpression of SpP5CS in Arabidopsis thaliana resulted in higher proline contents, longer roots, higher survival rates and less membrane damage under drought stress conditions compared with wild-type controls. SpP5CS-overexpressing A. thaliana was more resistant to drought stress than the wild type, whereas the deletion mutant sp5cs was less resistant to drought stress. Thus, SpP5CS may be a potential candidate target gene for increasing plant resistance to drought stress.
Keyword	Stipa purpurea P5CS proline drought tolerance INDUCED PROLINE ACCUMULATION PLAY DISTINCT ROLES 1ST 2 STEPS DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE SALT STRESS CONFERS TOLERANCE EXPRESSION BIOSYNTHESIS L. DEGRADATION
DOI	10.3390/ijms22179599
WOS ID	WOS:000694308500001
Citation statistics
Document Type	期刊论文
Identifier	http://ir.kib.ac.cn/handle/151853/73139
Collection	中国科学院昆明植物研究所
Affiliation	1.Chinese Acad Sci, Plant Germplasm & Genom Ctr, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Inst Tibetan Plateau Res Kunming, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100085, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Yunnan Populat & Family Planning Sci & Technol Re, Kunming 650021, Yunnan, Peoples R China
Recommended Citation GB/T 7714	Yang,Danni,Ni,Ruize,Yang,Shihai,et al. Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(17):9599.
APA	Yang,Danni.,Ni,Ruize.,Yang,Shihai.,Pu,Yanan.,Qian,Min.,...&Yang,Yongping.(2021).Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(17),9599.
MLA	Yang,Danni,et al."Functional Characterization of the Stipa purpurea P5CS Gene under Drought Stress Conditions".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.17(2021):9599.

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