An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum

doi:10.3390/horticulturae7090298

KIB OpenIR

	An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum
	Yao,Linling ; Huang,Jialin; Zhang,Shibao
	2021
发表期刊	HORTICULTURAE
ISSN	2311-7524
卷号	7 期号:9 页码:298
摘要	Paphiopedilum tigrinum is an endangered orchid with high ornamental value. However, seed germination and seedling regeneration in P. tigrinum is very difficult in vitro. Little is known about why P. tigrinum seedlings are difficult to propagate or how to improve the seed germination and seedling rates of this species. In this study, we investigated the developmental process of P. tigrinum from asymbiotic seed germination to seedling rooting by comparing it with P. appletoniantum, a much easier species for germination and seedling formation. We found that asymbiotic seed germination in P. tigrinum is limited by severe browning of the protocorm at the seed germination stage, and protocorm rooting at the differentiation stage was also proved to be difficult. The optimal medium for seed germination of P. tigrinum was a modified Harvais (mHa) medium supplemented with 0.5 mg center dot L-1 kinetin (Kin), 0.1 g center dot L-1 activated charcoal (AC) and 100 mL center dot L-1 coconut water (CW). At the protocorm differentiation stage, seedlings with 1-2 leaves were obtained on a 1/4 MS medium supplemented with 1.0 mg center dot L-1 6-benzylaminopurin (BA), 0.3 g center dot L-1 AC and 50-100 mL center dot L-1 CW after culturing for 120 day. At the seedling subculture stage, a 1/2 MS medium supplemented with 0.5-1.5 g center dot L-1 AC and 100 mL center dot L-1 CW was better for leaf and root growth of P. tigrinum. At the rooting stage, a 1/2 MS medium supplemented with 1.0 g center dot L-1 AC, 0.5 g center dot L-1 dolomite flour, 15 g center dot L-1 potato homogenate and 30 g center dot L-1 banana homogenate was most suitable for the growth and rooting of seedlings. This study has established an effective protocol for seed germination and seedling regeneration of P. tigrinum.
关键词	basal media endangered orchid germplasm conservation and utilization in vitro propagation IN-VITRO PROPAGATION LADYS SLIPPER ORCHID ACTIVATED-CHARCOAL PLANT-REGENERATION CULTURE MULTIPLICATION ESTABLISHMENT DELENATII MEDIA
DOI	10.3390/horticulturae7090298
WOS记录号	WOS:000699605400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73067
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Acad Life Sci, Beijing 100049, Peoples R China 3.Yuxi Normal Univ, Sch Chem Biol & Environm, Yuxi 653100, Peoples R China
推荐引用方式 GB/T 7714	Yao,Linling,Huang,Jialin,Zhang,Shibao. An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum[J]. HORTICULTURAE,2021,7(9):298.
APA	Yao,Linling,Huang,Jialin,&Zhang,Shibao.(2021).An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum.HORTICULTURAE,7(9),298.
MLA	Yao,Linling,et al."An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum".HORTICULTURAE 7.9(2021):298.