The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids

doi:10.1038/s41438-021-00471-9

KIB OpenIR

	The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids
	Dong,Shanshan ; Liu,Min ; Liu,Yang ; Chen,Fei ; Yang,Ting ; Chen,Lu ; Zhang,Xingtan ; Guo,Xing ; Fang,Dongming ; Li,Linzhou ; Deng,Tian ; Yao,Zhangxiu ; Lang,Xiaoan ; Gong,Yiqing ; Wu,Ernest ; Wang,Yaling ; Shen,Yamei ; Gong,Xun; Liu,Huan ; Zhang,Shouzhou
	2021
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	8 期号:1 页码:38
摘要	Magnolia biondii Pamp. (Magnoliaceae, magnoliids) is a phylogenetically, economically, and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China. Determining the genome sequence of M. biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus. We assembled a chromosome-level reference genome of M. biondii using similar to 67, similar to 175, and similar to 154Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing, 10X Genomics Chromium, and Hi-C scaffolding strategies, respectively. The final genome assembly was similar to 2.22Gb, with a contig N50 value of 269.11kb and a BUSCO complete gene percentage of 91.90%. Approximately 89.17% of the genome was organized into 19 chromosomes, resulting in a scaffold N50 of 92.86Mb. The genome contained 47,547 protein-coding genes, accounting for 23.47% of the genome length, whereas 66.48% of the genome length consisted of repetitive elements. We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales. Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites, plant-pathogen interactions, and responses to stimuli, which may improve the ecological fitness and biological adaptability of the lineage. Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae, which are sister to a clade comprising monocots and eudicots. The genome sequence of M. biondii could lead to trait improvement, germplasm conservation, and evolutionary studies on the rapid radiation of early angiosperms.
关键词	ALIGNMENT IDENTIFICATION PHYLOGENY TOOL ANGIOSPERMS IMPROVEMENT ANNOTATION SUPPLEMENT SEARCH PLANTS
DOI	10.1038/s41438-021-00471-9
WOS记录号	WOS:000624975000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73052
专题	中国科学院昆明植物研究所
作者单位	1.Fairy Lake Bot Garden, Lab Southern Subtrop Plant Divers, Shenzhen 518004, Peoples R China 2.Chinese Acad Sci, Shenzhen 518004, Peoples R China 3.BGI Shenzhen, State Key Lab Agr Genom, Shenzhen 518083, Peoples R China 4.Nanjing Forestry Univ, Nanjing 210037, Peoples R China 5.Fujian Agr & Forestry Univ, Fuzhou 350000, Peoples R China 6.Univ British Columbia, Vancouver, BC, Canada 7.Xian Bot Garden, Xian 710061, Peoples R China 8.Zhejiang Agr & Forestry Univ, Hangzhou 311300, Peoples R China 9.Chinese Acad Sci, Kunming Bot Garden, Kunming 650201, Yunnan, Peoples R China 10.Univ Copenhagen, Dept Biol, DK-2100 Copenhagen, Denmark
推荐引用方式 GB/T 7714	Dong,Shanshan,Liu,Min,Liu,Yang,et al. The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids[J]. HORTICULTURE RESEARCH,2021,8(1):38.
APA	Dong,Shanshan.,Liu,Min.,Liu,Yang.,Chen,Fei.,Yang,Ting.,...&Zhang,Shouzhou.(2021).The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids.HORTICULTURE RESEARCH,8(1),38.
MLA	Dong,Shanshan,et al."The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids".HORTICULTURE RESEARCH 8.1(2021):38.