Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana

doi:10.3390/md19030162

KIB OpenIR

	Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana
	Shi,Xiang ; Guan,Ruowei ; Zhou,Lutan ; Zuo,Zhichuang ; Tao,Xuelin ; Wang,Pin ; Zhou,Yanrong ; Yin,Ronghua ; Zhao,Longyan; Gao,Na ; Zhao,Jinhua
	2021
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	19 期号:3 页码:162
摘要	Unique fucosylated glycosaminoglycans (FG) have attracted increasing attention for various bioactivities. However, the precise structures of FGs usually vary in a species-specific manner. In this study, HfFG was isolated from Holothuria floridana and purified by anion exchange chromatography with the yield of similar to 0.9%. HfFG was composed of GlcA, GalNAc and Fuc, its molecular weight was 47.3 kDa, and the -OSO3-/-COO- molar ratio was 3.756. HfFG was depolymerized by a partial deacetylation-deaminative cleavage method to obtain the low-molecular-weight HfFG (dHfFG). Three oligosaccharide fragments (Fr-1, Fr-2, Fr-3) with different molecular weights were isolated from the dHfFG, and their structures were revealed by 1D and 2D NMR spectroscopy. HfFG should be composed of repeating trisaccharide units -{(L-FucS-alpha 1,3-)D-GlcA-beta 1,3-D-GalNAc(4S6S)-beta 1,4-}-, in which sulfated fucose (FucS) includes Fuc(2S4S), Fuc(3S4S) and Fuc(4S) residues linked to O-3 of GlcA in a ratio of 45:35:20. Furthermore, the heparanase inhibitory activities of native HfFG and oligosaccharide fragments (Fr-1, Fr-2, Fr-3) were evaluated. The native HfFG and its oligosaccharides exhibited heparanase inhibitory activities, and the activities increased with the increase of molecular weight. Additionally, structural characteristics such as sulfation patterns, the terminal structure of oligosaccharides and the presence of fucosyl branches may be important factors affecting heparanase inhibiting activity.
关键词	Holothuria floridana fucosylated glycosaminoglycans oligosaccharide chemical structure heparanase
DOI	10.3390/md19030162
WOS记录号	WOS:000633845000001
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73024
专题	中国科学院昆明植物研究所
作者单位	1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Guangxi Univ Chinese Med, Inst Marine Drugs, Nanning 530200, Peoples R China 4.South Cent Univ Nationalities, Natl Demonstrat Ctr Expt Ethnopharmacol Educ, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Shi,Xiang,Guan,Ruowei,Zhou,Lutan,et al. Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana[J]. MARINE DRUGS,2021,19(3):162.
APA	Shi,Xiang.,Guan,Ruowei.,Zhou,Lutan.,Zuo,Zhichuang.,Tao,Xuelin.,...&Zhao,Jinhua.(2021).Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana.MARINE DRUGS,19(3),162.
MLA	Shi,Xiang,et al."Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana".MARINE DRUGS 19.3(2021):162.