Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy

doi:10.3390/molecules26102858

KIB OpenIR

	Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy
	Feng,Jiling ; Mansouripour,Anahitasadat ; Xi,Zhichao ; Zhang,Li ; Xu,Gang; Zhou,Hua ; Xu,Hongxi
	2021
发表期刊	MOLECULES
ISSN	1420-3049
卷号	26 期号:10 页码:2858
摘要	Nujiangexanthone A (NJXA), a bioactive component isolated from the leaves of Garcinia nujiangensis, has been reported to exhibit anti-inflammatory, antioxidant, and antitumor effects. Our previous work has shown that NJXA induced G(0/1) arrest and apoptosis, thus suppressing cervical cancer cell growth. The present study provides new evidence that NJXA can induce cell death in HeLa cells by promoting mitophagy. We first identified that NJXA triggered GFP-LC3 and YFP-Parkin puncta accumulation, which are biomarkers of mitophagy. Moreover, NJXA degraded the mitochondrial membrane proteins Tom20 and Tim23 and mitochondrial fusion proteins MFN1 and MFN2, downregulated Parkin, and stabilized PINK1. Additionally, we revealed that NJXA induced lysosome degradation and colocalization of mitochondria and autophagosomes, which was attenuated by knocking down ATG7, the key regulator of mitophagy. Furthermore, since mitophagy is induced under starvation conditions, we detected the cytotoxic effect of NJXA in nutrient-deprived HeLa cells and observed better cytotoxicity. Taken together, our work contributes to the further clarification of the mechanism by which NJXA inhibits cervical cancer cell proliferation and provides evidence that NJXA has the potential to develop anticancer drugs.
关键词	nujiangexanthone A mitophagy ATG7 starvation MITOCHONDRIAL DYSFUNCTION GARCINIA-NUJIANGENSIS COMPOUND AUTOPHAGY NUJIANGEXATHONE ACTIVATION
DOI	10.3390/molecules26102858
WOS记录号	WOS:000655057000001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73003
专题	中国科学院昆明植物研究所
作者单位	1.Shanghai Univ Tradit Chinese Med, Inst Cardiovasc Dis Integrated Tradit Chinese & W, Shuguang Hosp, 528 Zhangheng Rd, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Sch Pharm, 1200 Cailun Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Feng,Jiling,Mansouripour,Anahitasadat,Xi,Zhichao,et al. Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy[J]. MOLECULES,2021,26(10):2858.
APA	Feng,Jiling.,Mansouripour,Anahitasadat.,Xi,Zhichao.,Zhang,Li.,Xu,Gang.,...&Xu,Hongxi.(2021).Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.MOLECULES,26(10),2858.
MLA	Feng,Jiling,et al."Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy".MOLECULES 26.10(2021):2858.