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Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura
Tang, Jinxiang1,3; Yang, Dahai2; Wu, Jianqiang1; Chen, Suiyun3; Wang, Lei1
Corresponding AuthorWang, Lei(leiwang@mail.kib.ac.cn)
2020-04-01
Source PublicationPLANT DIVERSITY
ISSN2096-2703
Volume42Issue:2Pages:111-119
AbstractJasmonic acid (JA) plays important roles in plant resistance to insect herbivores. One important derivative of JA is 12-OH-JA, which is produced by two independent pathways: direct hydroxylation of JA by jasmonate-induced oxygenases (JOXs) or hydrolyzation of 12-OH-JA-Ile.Yet the function of 12-OH-JA in plant-herbivore interactions remains largely unknown. In this study, we silenced four JOX homologs independently in the wild tobacco Nicotiana attenuata by virus-induced gene silencing (VIGS), and found that all four JOX homologs are involved in JA hydroxylation. Simultaneously silencing the four JA hydroxylases in VIGS-NaJOXs plants decreased herbivory-induced 12-OH-JA by 33%, but JA and JA-Ile levels increased by 45% and 30%, respectively, compared to those in control plants. Compared to direct hydroxylation from JA, hydrolyzation from 12-OH-JA-Ile is equally important for herbivory-induced 12-OH-JA accumulation: in the 12-OH-JA-Ile deficient irJAR4/6 plants, 12-OH-JA decreased 34%. Moreover, VIGS-NaJOXs plants exhibited enhanced resistance to the generalist herbivore Spodoptera litura. The poor larval performance was strongly correlated with high levels of several JA-Ile-dependent direct defense metabolites in the VIGS-NaJOXs plants. When we simultaneously silenced all four JA hydroxylases in the JA-Ile-deficient irJAR4/6 background, the enhanced herbivore resistance diminished, demonstrating that enhanced herbivore resistance resulted from elevated JA-Ile levels. Given that silencing these NaJOX-like genes did not detectably alter plant growth but highly increased plant defense levels, we propose that JOX genes are potential targets for genetic improvement of herbivore-resistant crops. Copyright (C) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
KeywordJA metabolism 12-OH-JA Spodoptera litura 2-Oxoglutarate oxygenase Herbivore defense Nicotiana attenuata
DOI10.1016/j.pld.2019.11.005
Indexed BySCI ; SCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000530163800006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/71668
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Acad Tobacco Agr Sci, Tobacco Breeding & Biotechnol Res Ctr, Natl Tobacco Genet Engn Res Ctr, Key Lab Tobacco Biotechnol Breeding, Kunming 650021, Yunnan, Peoples R China
3.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Tang, Jinxiang,Yang, Dahai,Wu, Jianqiang,et al. Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura[J]. PLANT DIVERSITY,2020,42(2):111-119.
APA Tang, Jinxiang,Yang, Dahai,Wu, Jianqiang,Chen, Suiyun,&Wang, Lei.(2020).Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura.PLANT DIVERSITY,42(2),111-119.
MLA Tang, Jinxiang,et al."Silencing JA hydroxylases in Nicotiana attenuata enhances jasmonic acid-isoleucine-mediated defenses against Spodoptera litura".PLANT DIVERSITY 42.2(2020):111-119.
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