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Development of Microsatellite Markers for a Dioecious Herpetospermum pedunculosum (Cucurbitaceae)
Chen, Zhu-Qing1; Zhou, Zhi-Li2; Wang, Lin-Lin2; Meng, Li-Hua1; Duan, Yuan-Wen2
Corresponding AuthorMeng, Li-Hua(menglihua@mails.ucas.ac.cn)
2020-02-01
Source PublicationEVOLUTIONARY BIOINFORMATICS
ISSN1176-9343
Volume16Pages:6
AbstractUnderstanding the evolution of flower diversity is a central topic in plant evolutionary ecology, and natural selection on floral traits via male fitness could be estimated quantitatively using microsatellites. Here, based on RNA sequencing, we developed simple sequence repeat primers and verified polymorphisms in 2 wild populations of Herpetospermum pedunculosum (Cucurbitaceae), a dioecious annual plants native to the Himalaya Mountains. A total of 131 paired primers were designed; 15 paired primers were found to be polymorphic, with the expected heterozygosity varying between 0.280 and 0.767. We also identified 58 genotypes in 20 plants from the 2 populations. Conclusively, these primers could be effective in examining male fitness and population genetic structure of H pedunculosum in future studies.
Keywordtranscriptome sequencing SSR paternity analysis male fitness floral traits
DOI10.1177/1176934320908261
Indexed BySCI ; SCI
Language英语
WOS Research AreaEvolutionary Biology ; Mathematical & Computational Biology
WOS SubjectEvolutionary Biology ; Mathematical & Computational Biology
WOS IDWOS:000517212600001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/71246
Collection中国西南野生生物种质资源库
Corresponding AuthorMeng, Li-Hua
Affiliation1.Yunnan Normal Univ, Key Lab Yunnan Biomass Energy & Biotechnol Enviro, Sch Life Sci, Kunming 650500, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhu-Qing,Zhou, Zhi-Li,Wang, Lin-Lin,et al. Development of Microsatellite Markers for a Dioecious Herpetospermum pedunculosum (Cucurbitaceae)[J]. EVOLUTIONARY BIOINFORMATICS,2020,16:6.
APA Chen, Zhu-Qing,Zhou, Zhi-Li,Wang, Lin-Lin,Meng, Li-Hua,&Duan, Yuan-Wen.(2020).Development of Microsatellite Markers for a Dioecious Herpetospermum pedunculosum (Cucurbitaceae).EVOLUTIONARY BIOINFORMATICS,16,6.
MLA Chen, Zhu-Qing,et al."Development of Microsatellite Markers for a Dioecious Herpetospermum pedunculosum (Cucurbitaceae)".EVOLUTIONARY BIOINFORMATICS 16(2020):6.
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