A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress
Yang, Yunqiang1,2,3; Pu, Yanan1,2,3; Yin, Xin1,2,3,4; Du, Jiancan1; Zhou, Zhili1,2,3; Yang, Danni1,2,3,4; Sun, Xudong1,2,3; Sun, Hang1,2,3; Yang, Yongping1,2,3
Corresponding AuthorYang, Yongping(yangyp@mail.kib.ac.cn)
2019-10-09
Source PublicationJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN0021-8561
Volume67Issue:40Pages:11077-11088
AbstractCuticular wax accumulation in plants contributes to drought tolerance. Here, we compared the drought levels on two varieties with different genotypes in turnip (Brassica rapa var. rapa) and found that the drought tolerance was higher in the waxy KTRG-B48a than in the wax-free KTRG-B48b. A combination of PacBio and Illumina sequencing analyses revealed that differential transcripts were mainly enriched in the wax synthesis pathway, and a splice variant (BrrWSD1-X2) was identified in the waxy KTRG-B48a. BrrWSD1-X2 had a stronger ability to synthesize wax esters than BrrWSD1-X1 using heterologous expression in yeast (Saccharomyces cerevisiae) mutant H1246a. Then, we speculated that the T to C transversion of the third intron and the higher number of TA repeats in the third intron of BrrWSD1 DNA in the waxy KTRG-B48a may result in a lower efficiency of splicing recognition of the third intron, resulting in the emergence of BrrWSD1-X2 in waxy varieties.
Keywordwax esters drought stress alternative splicing turnip BrrWSD1
DOI10.1021/acs.jafc.9b04069
Indexed BySCI ; SCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000490354700009
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/69965
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorYang, Yongping
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Inst Tibetan Plateau Res Kunming, Kunming 650201, Yunnan, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yunqiang,Pu, Yanan,Yin, Xin,et al. A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(40):11077-11088.
APA Yang, Yunqiang.,Pu, Yanan.,Yin, Xin.,Du, Jiancan.,Zhou, Zhili.,...&Yang, Yongping.(2019).A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(40),11077-11088.
MLA Yang, Yunqiang,et al."A Splice Variant of BrrWSD1 in Turnip (Brassica rapa var. rapa) and Its Possible Role in Wax Ester Synthesis under Drought Stress".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.40(2019):11077-11088.
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