Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)

doi:10.1002/aps3.1213

	Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
	Ye, Jun-Wei 1,2; Li, De-Zhu1
通讯作者	Li, De-Zhu(dzl@mail.kib.ac.cn)
	2019-08-01
发表期刊	APPLICATIONS IN PLANT SCIENCES
ISSN	2168-0450
卷号	7 期号:8 页码:4
摘要	Premise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species.
关键词	chloroplast microsatellite Lauraceae Lindera aggregata wuyao
DOI	10.1002/aps3.1213
收录类别	SCI
语种	英语
WOS记录号	WOS:000482942400003
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/68821
专题	中国西南野生生物种质资源库
通讯作者	Li, De-Zhu
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.Shanghai Sci & Technol Museum, Shanghai Nat Hist Museum, Nat Hist Res Ctr, Shanghai 200127, Peoples R China
推荐引用方式 GB/T 7714	Ye, Jun-Wei,Li, De-Zhu. Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(8):4.
APA	Ye, Jun-Wei,&Li, De-Zhu.(2019).Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae).APPLICATIONS IN PLANT SCIENCES,7(8),4.
MLA	Ye, Jun-Wei,et al."Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)".APPLICATIONS IN PLANT SCIENCES 7.8(2019):4.

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