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Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)
Ye, Jun-Wei1,2; Li, De-Zhu1
Corresponding AuthorLi, De-Zhu(
AbstractPremise of the Study To investigate the population genetics and evolutionary history of wuyao (Lindera aggregata, Lauraceae) and other Lindera species, polymorphic chloroplast microsatellite (cpSSR) primers were developed. Methods and Results The complete chloroplast genome of L. glauca was used to design 74 primers for L. aggregata. Agarose gel electrophoresis and Sanger sequencing were used to select 20 polymorphic primers. These primers were then tested in 70 individuals from three populations of L. aggregata. The number of alleles ranged from two to four, and the unbiased haploid diversity index ranged from 0.457 to 0.685. Up to 17 primers successfully amplified in four other Lindera species: L. prattii, L. chunii, L. lungshengensis, and L. pulcherrima var. hemsleyana. Conclusions The 20 primers reported here can potentially be used for future genetic studies of L. aggregata and other Lindera species.
Keywordchloroplast microsatellite Lauraceae Lindera aggregata wuyao
Indexed BySCI
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000482942400003
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Document Type期刊论文
Corresponding AuthorLi, De-Zhu
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
2.Shanghai Sci & Technol Museum, Shanghai Nat Hist Museum, Nat Hist Res Ctr, Shanghai 200127, Peoples R China
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GB/T 7714
Ye, Jun-Wei,Li, De-Zhu. Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(8):4.
APA Ye, Jun-Wei,&Li, De-Zhu.(2019).Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae).APPLICATIONS IN PLANT SCIENCES,7(8),4.
MLA Ye, Jun-Wei,et al."Development of 20 chloroplast microsatellite primers in wuyao (Lindera aggregata, Lauraceae)".APPLICATIONS IN PLANT SCIENCES 7.8(2019):4.
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