Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants

doi:10.1016/j.pld.2019.04.003

	Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants
	Basak, Supriyo 1,4; Moolam, Ramesh Aadi 1; Parida, Ajay 2; Mitra, Sudip 3; Rangan, Latha 1
通讯作者	Rangan, Latha(Latha_rangan@yahoo.com)
	2019-06-01
发表期刊	PLANT DIVERSITY
ISSN	2096-2703
卷号	41 期号:3 页码:206-211
摘要	Accurate detection of unique herbs is crucial for herbal medicine preparation. Zingiberaceae species, which are important in Ayurvedic medicine of India, are often misidentified in Northeast (NE) Indian herbal markets. Kaempferia galanga (Zingiberaceae) is one of the major components of popular Ayurvedic drugs used for rheumatic diseases (i.e., "Gandha Thailam" and "Rasnairandadi Kashayam"), contusions, fractures, and sprains. In NE India, herbal healers often misidentify plants from the Marantaceae family (e.g., Calathea bachemiana and Maranta leuconeura) as Kaempferia, which leads to adulteration of the medicinal herb. This misidentification of herbs occurs in NE India because Zingiberaceae plant barcoding information is inadequate. As a consequence, herbal medicine is not only therapeutically less effective but may also cause adverse reactions that range from mild to life-threatening. In this study, we used eight barcoding loci to develop "fingerprints" for four Kaempferia species and two species frequently mistaken for Kaempferia. The PCR and sequencing success of the loci matK, rbcL and trnH-psbA were found to be 100%; the combination of matK, rbcL, and trnH-psbA proved to be the ideal locus for discriminating the Kaempferia species from their adulterants because the combined loci showed greater variability than individual loci. This reliable tool was therefore developed in the current study for accurate identification of Kaempferia plants which can effectively resolve identification issues for herbal healers. Copyright (C) 2019 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
关键词	Ayurveda Barcoding Chloroplast Molecular authentication Zingiberaceae Calathea bachemiana
DOI	10.1016/j.pld.2019.04.003
收录类别	SCI
语种	英语
WOS记录号	WOS:000480443600008
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/68368
专题	中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Rangan, Latha
作者单位	1.Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Gauhati 781039, Assam, India 2.Inst Life Sci, NALCO Nagar Rd,NALCO Sq, Bhubaneswar 751023, India 3.Indian Inst Technol Guwahati, Ctr Rural Technol, Gauhati 781039, Assam, India 4.Chinese Acad Sci, Key Lab Plant Divers & Biogeog East Asia, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Basak, Supriyo,Moolam, Ramesh Aadi,Parida, Ajay,et al. Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants[J]. PLANT DIVERSITY,2019,41(3):206-211.
APA	Basak, Supriyo,Moolam, Ramesh Aadi,Parida, Ajay,Mitra, Sudip,&Rangan, Latha.(2019).Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants.PLANT DIVERSITY,41(3),206-211.
MLA	Basak, Supriyo,et al."Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants".PLANT DIVERSITY 41.3(2019):206-211.

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