Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)
Ji, Yunheng1,2; Liu, Changkun1; Yang, Zhenyan1; Yang, Lifang1,3; He, Zhengshan4; Wang, Hengchang5; Yang, Junbo4; Yi, Tingshuang4
Corresponding AuthorJi, Yunheng(jiyh@mail.kib.ac.cn) ; Yang, Junbo(jbyang@mail.kib.ac.cn) ; Yi, Tingshuang(tingshuangyi@mail.kib.ac.cn)
2019-07-25
Source PublicationMOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
Pages13
AbstractComplete plastid genome (plastome) sequences and nuclear ribosomal DNA (nrDNA) regions have been proposed as candidates for the next generation of DNA barcodes for plant species discrimination. However, the efficacy of this approach still lacks comprehensive evaluation. We carried out a case study in the economically important but phylogenetically and taxonomically difficult genus Panax (Araliaceae). We generated a large data set of plastomes and nrDNA sequences from multiple accessions per species. Our data improved the phylogenetic resolution and levels of species discrimination in Panax, compared to any previous studies using standard DNA barcodes. This provides new insights into the speciation, lineage diversification and biogeography of the genus. However, both plastome and nrDNA failed to completely resolve the phylogenetic relationships in the Panax bipinnatifidus species complex, and only half of the species within it were recovered as monophyletic units. The results suggest that complete plastome and ribosomal DNA sequences can substantially increase species discriminatory power in plants, but they are not powerful enough to fully resolve phylogenetic relationships and discriminate all species, particularly in evolutionarily young and complex plant groups. To gain further resolving power for closely related species, the addition of substantial numbers of nuclear markers is likely to be required.
Keywordnext-generation DNA barcodes Panax plastome ribosomal DNA species discrimination standard DNA barcodes
DOI10.1111/1755-0998.13050
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000479384000001
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68276
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorJi, Yunheng; Yang, Junbo; Yi, Tingshuang
Affiliation1.Chinese Acad Sci, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming Inst Bot, Kunming, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming, Yunnan, Peoples R China
3.Yunnan Univ, Sch Life Sci, Kunming, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
5.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Ji, Yunheng,Liu, Changkun,Yang, Zhenyan,et al. Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)[J]. MOLECULAR ECOLOGY RESOURCES,2019:13.
APA Ji, Yunheng.,Liu, Changkun.,Yang, Zhenyan.,Yang, Lifang.,He, Zhengshan.,...&Yi, Tingshuang.(2019).Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae).MOLECULAR ECOLOGY RESOURCES,13.
MLA Ji, Yunheng,et al."Testing and using complete plastomes and ribosomal DNA sequences as the next generation DNA barcodes in Panax (Araliaceae)".MOLECULAR ECOLOGY RESOURCES (2019):13.
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