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Pollination-Induced Transcriptome and Phylogenetic Analysis in Cymbidium tortisepalum (Orchidaceae)
Zhao, Y.1; Li, D.2; Liu, T.1
Corresponding AuthorZhao, Y.(yhzhao808@163.com)
2019-07-01
Source PublicationRUSSIAN JOURNAL OF PLANT PHYSIOLOGY
ISSN1021-4437
Volume66Issue:4Pages:618-627
AbstractOrchids comprise a group of ecologically and evolutionarily significant plants, and the Orchidaceae is one of the most abundant angiosperm families. Yet little is understood about the genetic control of its flower development. Like many orchids, Cymbidium tortisepalum ovaries develop only following pollination. In this study, high-throughput RNA-seq technology was used to characterize the C. tortisepalum transcriptome and to identify differentially expressed genes during floral development. A total number of 45165976 reads were generated and assembled into 60301 unigenes. 38643 unigenes matched to proteins in the NCBI database. To compare the transcriptomes of pre-pollinated and post-pollinated gynostemia, an RNA-seq method was used. RNA-seq abundance analysis identified potentially all of the transcribed genes in this species' gynostemium before and after pollination, including 488943 in the pre-pollinated gynostemium transcriptome, and 88829transcripts in the post-pollinated gynostemium. Further comparison of the transcripts revealed that 7252genes had either been up-regulated or down-regulated in post-pollinated compared with pre-pollinated gynostemium development. The preponderance of auxin and ethylene biosynthesis and signaling pathway factors in these transcriptomes suggested that the early pollination-induced ovary development involves these two hormones, as has been observed in other species. The C. tortisepalum gynostemium RNA-sequencing data were compared to transcriptome data of Oryza sativa and Arabidopsis thaliana, including pre-pollination and post-pollination stages and total 11 conserved orthologs related to protein kinase were found by phylogenetic analysis. Therefore, the sequence and expression data produced from this study provides the most comprehensive sequence resource available for C. tortisepalum study to date.
KeywordCymbidium tortisepalum ovary development phylogenetic analysis pollination transcriptome
DOI10.1134/S1021443719040174
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000475714400013
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68124
Collection中国西南野生生物种质资源库
Corresponding AuthorZhao, Y.
Affiliation1.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650204, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Y.,Li, D.,Liu, T.. Pollination-Induced Transcriptome and Phylogenetic Analysis in Cymbidium tortisepalum (Orchidaceae)[J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,2019,66(4):618-627.
APA Zhao, Y.,Li, D.,&Liu, T..(2019).Pollination-Induced Transcriptome and Phylogenetic Analysis in Cymbidium tortisepalum (Orchidaceae).RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,66(4),618-627.
MLA Zhao, Y.,et al."Pollination-Induced Transcriptome and Phylogenetic Analysis in Cymbidium tortisepalum (Orchidaceae)".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 66.4(2019):618-627.
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