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Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)
Li, Wenjun1; Su, Zhihao1; Li, Airong2; Guan, Kaiyun1,2; Feng, Ying1
Corresponding AuthorFeng, Ying(luckfy@ms.xjb.ac.cn)
2019-04-01
Source PublicationGRASSLAND SCIENCE
ISSN1744-6961
Volume65Issue:2Pages:135-138
AbstractTo quantify the population-level genetic characteristics of Pedicularis kansuensis, polymorphic microsatellite loci (simple sequence repeats) were identified and characterized. Methods and Results: Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified 1594 sequences by four softwares simultaneously. We developed and characterized 18 polymorphic microsatellite primers for P. kansuensis using 45 individuals that were collected from three P. kansuensis populations. The number of alleles varied from two to 12. The observed heterozygosity and expected heterozygosity per locus ranged from 0.182 to 0.933 and 0.227 to 0.904 in the pooled population, respectively. All of the primers that were developed were also successfully applied in Pedicularis cheilanthifolia and Pedicularis violascens. These markers should be useful in probing the genetic diversity, genetic structure of P. kansuensis, which could provide information about assessment and prediction of potential risks of this species in its distribution areas.
Keywordinvasive native species Pedicularis kansuensis polymorphic microsatellites
DOI10.1111/grs.12222
Indexed BySCI
Language英语
WOS IDWOS:000462884700008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/67683
Collection中国西南野生生物种质资源库
Corresponding AuthorFeng, Ying
Affiliation1.Chinese Acad Sci, Key Lab Biogeog & Bioresource Arid Land, Xinjiang Inst Ecol & Geog, Urumqi, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Res & Dev Wild Plant Resources, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Li, Wenjun,Su, Zhihao,Li, Airong,et al. Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)[J]. GRASSLAND SCIENCE,2019,65(2):135-138.
APA Li, Wenjun,Su, Zhihao,Li, Airong,Guan, Kaiyun,&Feng, Ying.(2019).Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae).GRASSLAND SCIENCE,65(2),135-138.
MLA Li, Wenjun,et al."Isolation and characterization of 18 microsatellites for the invasive native Pedicularis kansuensis (Orobanchaceae)".GRASSLAND SCIENCE 65.2(2019):135-138.
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