Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean

doi:10.3390/ijms20061282

	Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean
	Yu, Anmin 1,2; Wang, Zaiqing 1,2; Zhang, Yang 3; Li, Fei 1; Liu, Aizhong4
通讯作者	Liu, Aizhong(liuaizhong@mail.kib.ac.cn)
	2019-03-02
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
卷号	20 期号:6 页码:21
摘要	The physiological and molecular basis of seed size formation is complex, and the development of seed coat (derived from integument cells) might be a critical factor that determines seed size formation for many endospermic seeds. Castor bean (Ricinus communis L.), a model system of studying seed biology, has large and persistent endosperm with a hard seed coat at maturity. Here, we investigated the potential molecular mechanisms underlying seed size formation in castor bean by comparing the difference between global gene expression within developing seed coat tissues between the large-seed ZB107 and small-seed ZB306. First, we observed the cell size of seed coat and concluded that the large seed coat area of ZB107 resulted from more cell numbers (rather than cell size). Furthermore, we found that the lignin proportion of seed coat was higher in ZB306. An investigation into global gene expression of developing seed coat tissues revealed that 815 genes were up-regulated and 813 were down-regulated in ZB306 relative to ZB107. Interestingly, we found that many genes involved in regulating cell division were up-regulated in ZB107, whereas many genes involved in regulating lignin biosynthesis (including several NAC members, as well as MYB46/83 and MYB58/63) and in mediating programmed cell death (such as CysEP1 and VPE) were up-regulated in ZB306. Furthermore, the expression patterns of the genes mentioned above indicated that the lignification of seed coat tissues was enhanced and occurred earlier in the developing seeds of ZB306. Taken together, we tentatively proposed a potential scenario for explaining the molecular mechanisms of seed coat governing seed size formation in castor bean by increasing the cell number and delaying the onset of lignification in seed coat tissues in large-seed ZB107. This study not only presents new information for possible modulation of seed coat related genes to improve castor seed yield, but also provides new insights into understanding the molecular basis of seed size formation in endospermic seeds with hard seed coat.
关键词	castor bean seed coat seed size lignin cell division
DOI	10.3390/ijms20061282
收录类别	SCI
语种	英语
WOS记录号	WOS:000465523400009
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/67501
专题	中国西南野生生物种质资源库
通讯作者	Liu, Aizhong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jiangxi Acad Agr Sci, Crops Res Inst, Jiangxi Prov Key Lab Oil Crops Biol, Nanchang 330200, Jiangxi, Peoples R China 4.Southwest Forestry Univ, Minist Educ, Key Lab Forest Resources Conservat & Utilizat Sou, Kunming 650224, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Anmin,Wang, Zaiqing,Zhang, Yang,et al. Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(6):21.
APA	Yu, Anmin,Wang, Zaiqing,Zhang, Yang,Li, Fei,&Liu, Aizhong.(2019).Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(6),21.
MLA	Yu, Anmin,et al."Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.6(2019):21.

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