Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway

doi:10.1016/j.fitote.2019.04.008

	Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway
	Yang, Zhenyan 1,2; Yang, Lifang 1,4; Liu, Changkun 1,2; Qin, Xujie3 ; Liu, Haiyang3 ; Chen, Jiahui1 ; Ji, Yunheng1,2
通讯作者	Chen, Jiahui(chenjh@mail.kib.ac.cn) ; Ji, Yunheng(jiyh@mail.kib.ac.cn)
	2019-06-01
发表期刊	FITOTERAPIA
ISSN	0367-326X
卷号	135 页码:52-63
摘要	Steroidal saponins, one of the most diverse groups of plant-derived natural products, elicit biological and pharmacological activities; however, the genes involved in their biosynthesis and the corresponding biosynthetic pathway in monocotyledon plants remain unclear. This study aimed to identify genes involved in the biosynthesis of steroidal saponins by performing a comparative analysis among transcriptomes of Paris polyphylla var. chinensis (PPC), Ypsilandra thibetica (YT), and Polygonatum kingianum (PK). De novo transcriptome assemblies generated 57,537, 140,420, and 151,773 unigenes from PPC, YT, and PK, respectively, of which 56.54, 47.81, and 44.30% were successfully annotated, respectively. Among the transcriptomes for PPC, YT, and PK, we identified 194, 169, and 131; 17, 14, and 26; and, 80, 122, and 113 unigenes corresponding to terpenoid backbone biosynthesis; sesquiterpenoid and triterpenoid biosynthesis; and, steroid biosynthesis pathways, respectively. These genes are putatively involved in the biosynthesis of cholesterol that is the primary precursor of steroidal saponins. Phylogenetic analyses indicated that lanosterol synthase may be exclusive to dicotyledon plant species, and the cytochrome P450 unigenes were closely related to clusters CYP90B1 and CYP734A1, which are UDP-glycosyltransferases unigenes homologous with the UGT73 family. Thus, unigenes of beta-glucosidase may be candidate genes for catalysis of later period modifications of the steroidal saponin skeleton. Our data provide evidence to support the hypothesis that monocotyledons biosynthesize steroidal saponins from cholesterol via the cycloartenol pathway.
关键词	Steroidal saponins Biosynthesis Transcriptome Monocotyledons Cholesterol Cycloartenol pathway
DOI	10.1016/j.fitote.2019.04.008
收录类别	SCI
语种	英语
WOS记录号	WOS:000470048000009
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/67406
专题	中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Chen, Jiahui; Ji, Yunheng
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Yunnan Univ, Sch Life Sci, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yang, Zhenyan,Yang, Lifang,Liu, Changkun,et al. Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway[J]. FITOTERAPIA,2019,135:52-63.
APA	Yang, Zhenyan.,Yang, Lifang.,Liu, Changkun.,Qin, Xujie.,Liu, Haiyang.,...&Ji, Yunheng.(2019).Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.FITOTERAPIA,135,52-63.
MLA	Yang, Zhenyan,et al."Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway".FITOTERAPIA 135(2019):52-63.

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