Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis

doi:10.1016/j.pld.2018.11.002

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	Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis
	Jia, Yanxia; Li, Weiqi
通讯作者	Li, Weiqi(weiqili@mail.kib.ac.cn)
	2018-12-01
发表期刊	PLANT DIVERSITY
ISSN	2096-2703
卷号	40 期号:6 页码:292-298
摘要	Storage oil breakdown plays an important role in the life cycle of many plants by providing the carbon skeletons that support seedling growth immediately following germination. 1-Butanol, a specific inhibitor of phospholipase D (PLD)-dependent production of the signalling molecule phosphatidic acid (PA), inhibited Arabidopsis seed germination. N-Acylethanolamines (NAEs), which have been shown to inhibits PLD alpha 1 activity, have no effect on seed germination. However, mobilization profile of triacylglycerols (TAG) that induced by each compound has not been reported. To gain deeper insights into the mode of mobilization of TAG during NAE 12:0 or 1-butanol treatment, we conducted a detailed comparative analysis of the effect of NAE 12:0, DMSO, 1-butanol and tert-butanol on Arabidopsis seed germination and fatty acid composition, tert-butanol and DMSO served as the corresponding controls treatment respectively. Our data show that 1-butanol, but not the inactive tert-butanol isomer, inhibited Arabidopsis seed germination, which is accompanied by a with retardation of the mobilization of triacylglycerols (TAG). In contrast, NAE 12:0 did not affect mobilization of TAG, nor did it significantly delay seed germination as monitored by radicle and cotyledon emergence. 1-Butanol induced RNA degradation in seeds and seedlings. We speculate that the large-scale degradation of RNA under the induction of 1-butanol may lead to abnormal gene expression in genes necessary for seed germination, including the genes needed for the mobilization of oil bodies, and thus cause a delay of seed germination. To the best of our knowledge, we report for the first time that 1-butanol delays the mobilization of TAG. Copyright (c) 2018 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
DOI	10.1016/j.pld.2018.11.002
收录类别	SCI
语种	英语
WOS记录号	WOS:000454560900005
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/66545
专题	Plant Diversity
通讯作者	Li, Weiqi
作者单位	Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yanxia,Li, Weiqi. Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis[J]. PLANT DIVERSITY,2018,40(6):292-298.
APA	Jia, Yanxia,&Li, Weiqi.(2018).Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis.PLANT DIVERSITY,40(6),292-298.
MLA	Jia, Yanxia,et al."Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis".PLANT DIVERSITY 40.6(2018):292-298.