Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.

KIB OpenIR

	Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.
	Li, Shun; Huang, Jun-Chao
	2018
发表期刊	Natural products and bioprospecting
ISSN	2192-2195
卷号	8 期号:5 页码:397-403
摘要	Astaxanthin is a value-added ketocarotenoid with great potential in nutraceutical and pharmaceutical industries. Genetic engineering of heterologous hosts for astaxanthin production has attracted great attention. In this study, we assessed some key factors, including codon usage of the expressed genes, types of promoters, bacterial strains, and culture media, for engineered Escherichia coli to produce astaxanthin. The effect of codon usage was shown to be related to the types of promoters. E. coli DH5alpha was superior to other strains for astaxanthin production. Different culture media greatly affected the contents and yields of astaxanthin in engineered E. coli. When the expression cassette containing GadE promoter and its driving genes, HpCHY and CrBKT, was inserted into the plasmid pACCAR16DeltacrtX and expressed in E. coli DH5alpha, the engineered strain was able to produce 4.30±0.28mg/g dry cell weight (DCW) or 24.16±2.03mg/L of astaxanthin, which was a sevenfold or 40-fold increase over the initial production of 0.62±0.03mg/g DCW or 0.61±0.05mg/L.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64786
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Li, Shun,Huang, Jun-Chao. Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.[J]. Natural products and bioprospecting,2018,8(5):397-403.
APA	Li, Shun,&Huang, Jun-Chao.(2018).Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin..Natural products and bioprospecting,8(5),397-403.
MLA	Li, Shun,et al."Assessment of Expression Cassettes and Culture Media for Different Escherichia coli Strains to Produce Astaxanthin.".Natural products and bioprospecting 8.5(2018):397-403.