Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L.

KIB OpenIR

	Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L.
	Wang, Zaiqing; Yang, Cuiling; Chen, Hao; Wang, Pei; Wang, Pengtao; Song, Chunpeng; Zhang, Xiao; Wang, Daojie
	2018
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	274 页码:410-419
摘要	Rapeseed (Brassica napus L.) is an important oil crop worldwide. For current B. napus production, it is urgent to develop new varieties with higher seed productivity and increased stress tolerance for better adaptation to the abiotic stresses as a result of global climate change. Genetic engineering, to some extent, can overcome the limitations of genetic exchange in conventional breeding. Consequently, it considered as an effective method for improving modern crop breeding for B. napus. Since crop stress resistance is a polygenic complex trait, only by multi-gene synergistic effects can effectively achieve the comprehensive stress resistance of crops. Hence, in the present study, five stress resistance genes, NCED3, ABAR, CBF3, LOSS, and ICE1 were transferred into B. napus. Compared with wildtype (WT) plants, the multi-gene transformants K15 exhibited pronounced growth advantage under both normal growth and stress conditions. Additionally, K15 plants also showed significantly higher resistance response to multiple stresses at seed germination and seedling stages than WT plants. Furthermore, K15 plants had significantly higher leaf temperature and significantly lower stomatal aperture and water loss rate than WT plants, which indicated that the water-holding capacity of K15 plants was significantly superior to that of WT plants after stress treatment. In addition, K15 plants had significantly higher abscisic acid (ABA) content and significantly lower malondialdehyde (MDA) content than WT plants. In conclusion, the above results suggested that multi-gene co-expression could rapidly trigger plant stress resistance, reduce the stress injury on plants and synergistically improve the comprehensive resistance of B. napus.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64692
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang, Zaiqing,Yang, Cuiling,Chen, Hao,et al. Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L.[J]. PLANT SCIENCE,2018,274:410-419.
APA	Wang, Zaiqing.,Yang, Cuiling.,Chen, Hao.,Wang, Pei.,Wang, Pengtao.,...&Wang, Daojie.(2018).Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L..PLANT SCIENCE,274,410-419.
MLA	Wang, Zaiqing,et al."Multi-gene co-expression can improve comprehensive resistance to multiple abiotic stresses in Brassica napus L.".PLANT SCIENCE 274(2018):410-419.