Optimization of extraction and analytical protocol for mass spectrometry-based metabolomics analysis of hepatotoxicity

KIB OpenIR

	Optimization of extraction and analytical protocol for mass spectrometry-based metabolomics analysis of hepatotoxicity
	Yang, Rui; Zhao, Qi; Hu, Dan-Dan; Xiao, Xue-Rong; Li, Fei
	2018
发表期刊	BIOMEDICAL CHROMATOGRAPHY
ISSN	0269-3879
卷号	32 期号:12 页码:e4359
摘要	Drug-induced liver injury is a clinically leading side-effect of drugs. In the present study, a liquid chromatography mass spectrometry-based metabolomics protocol was optimized for extraction and analysis of endogenous metabolites from liver tissue during hepatotoxicity. Various extraction solutions, resuspension solutions, extraction folds and dissolution methods for the supernatant were compared using the number of extracted total ions, relative response and relative extraction efficiency of targeted metabolites from liver tissue. The polar and nonpolar endogenous metabolites associated with liver injury were analyzed by hydrophilic interaction chromatography and reversed-phase liquid chromatography with UPLC-QTOFMS. The results indicated that extraction with 10-fold 50% acetonitrile in water and the supernatant diluted (1:1) with 100% acetonitrile rather than resuspension was the optimal extraction protocol. Subsequently, the optimized method was able to examine the change in metabolites in mouse liver tissue resulting from treatment with a toxic natural product, toosendanin. Taken together, the optimized extraction and analytical protocol provides high reliability and reproducibility for polar and nonpolar metabolites in liver tissue and may be suitable for metabolomics analysis of liver injury induced by drugs or chemicals.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64672
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yang, Rui,Zhao, Qi,Hu, Dan-Dan,et al. Optimization of extraction and analytical protocol for mass spectrometry-based metabolomics analysis of hepatotoxicity[J]. BIOMEDICAL CHROMATOGRAPHY,2018,32(12):e4359.
APA	Yang, Rui,Zhao, Qi,Hu, Dan-Dan,Xiao, Xue-Rong,&Li, Fei.(2018).Optimization of extraction and analytical protocol for mass spectrometry-based metabolomics analysis of hepatotoxicity.BIOMEDICAL CHROMATOGRAPHY,32(12),e4359.
MLA	Yang, Rui,et al."Optimization of extraction and analytical protocol for mass spectrometry-based metabolomics analysis of hepatotoxicity".BIOMEDICAL CHROMATOGRAPHY 32.12(2018):e4359.