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Systematic characterization and quantification of Rubiaceae-type cyclopeptides in 20 Rubia species by ultra performance liquid chromatography tandem mass spectrometry combined with chemometrics
Zhang, Xuejia1,2; Bi, Qirui1; Wu, Xingdong1; Wang, Zhe1; Miao, Yuanyuan1; Tan, Ninghua1,2
通讯作者Miao, Yuanyuan(miaoyuanyuan1989@163.com) ; Tan, Ninghua(nhtan@cpu.edu.cn)
2018-12-21
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1581页码:43-54
摘要Rubiaceae-type cyclopeptides (RAs) are one of characteristic constituents isolated from Rubia species, which are candidates of innovative anti-tumor drugs due to their significant bioactivity. However, approaches on the systematic characterization and quantification of RAs are still not available because of low contents and complicated purification. In this study, an ultra performance liquid chromatography coupled triple quadrupole tandem mass spectrometry (UPLC-QqQ-MS/MS) method was established and validated for qualitative and quantitative analysis of 14 RAs (1-14) in 20 Rubia plants from China. The separation was achieved on a Waters ACQUITY UPLC (R) BEH C-18 column (50 x 2.1 mm, 1.7 mu m) using gradient elution with a mobile phase consisting of 0.1% formic acid in acetonitrile and water. Multiple reaction monitoring (MRM) in positive mode was used to enable the selective detection of RAs from the Rubia root and rhizome extracts within 10 min. This method was proved to be specific, sensitive, precise, and accurate with the limits of detection and quantification at 0.6-11.4 ng/mL and 1.9-34.2 ng/mL, respectively, and the relative standard deviation (RSD) of the overall intra-day and inter-day precision less than 5.24%. Satisfactory recovery was obtained from 83.80% to 111.77%, with the RSD less than 5.32%. Totally, 67 samples were then qualitatively and quantitatively analyzed by this method and 51 of them were proved to contain RAs. Thereinto, R. podantha and R. yunnanensis from Yunnan were the two most abundant species. Additionally, RAs were detected in 8 Rubia species for the first time. Then chemometric approaches were revealed to explain the relationship between samples based on their contents of RAs. This study demonstrated that the method was not only useful for RAs source discovery and chemotaxonomy of Rubia species, but also could be extended to standardization of RAs medical materials and their new drug research and development. (C) 2018 Elsevier B.V. All rights reserved.
关键词Rubiaceae-type cyclopeptides Rubia UPLC-QqQ-MS/MS Multiple reaction monitoring Chemometric approach Chemotaxonomy
DOI10.1016/j.chroma.2018.10.049
收录类别SCI
语种英语
WOS记录号WOS:000453490100006
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/64660
专题中国科学院昆明植物研究所
通讯作者Miao, Yuanyuan; Tan, Ninghua
作者单位1.China Pharmaceut Univ, Sch Tradit Chinese Pharm, Dept TCMs Pharmaceut, State Key Lab Nat Med, Nanjing 211198, Jiangsu, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
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Zhang, Xuejia,Bi, Qirui,Wu, Xingdong,et al. Systematic characterization and quantification of Rubiaceae-type cyclopeptides in 20 Rubia species by ultra performance liquid chromatography tandem mass spectrometry combined with chemometrics[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1581:43-54.
APA Zhang, Xuejia,Bi, Qirui,Wu, Xingdong,Wang, Zhe,Miao, Yuanyuan,&Tan, Ninghua.(2018).Systematic characterization and quantification of Rubiaceae-type cyclopeptides in 20 Rubia species by ultra performance liquid chromatography tandem mass spectrometry combined with chemometrics.JOURNAL OF CHROMATOGRAPHY A,1581,43-54.
MLA Zhang, Xuejia,et al."Systematic characterization and quantification of Rubiaceae-type cyclopeptides in 20 Rubia species by ultra performance liquid chromatography tandem mass spectrometry combined with chemometrics".JOURNAL OF CHROMATOGRAPHY A 1581(2018):43-54.
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