Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing
Wang, Xinjia1,2; Zhong, Li1,2; Wu, Zhikun3; Sun, Huaying1; Wang, Hong1; Li, Dezhu4; Barrett, Spencer C. H.5; Zhou, Wei4
2019
Source PublicationAPPLICATIONS IN PLANT SCIENCES
ISSN2168-0450
Volume7Issue:1Pages:5
AbstractPremise of the Study Microsatellite markers were developed for Primula sinolisteri, a perennial distylous herb belonging to section Obconicolisteri (Primulaceae), to facilitate future investigations of the population genetics and mating patterns of populations in this species. Methods and Results We developed 30 microsatellite markers for P. sinolisteri using HiSeq X-Ten sequencing and measured polymorphism and genetic diversity in a sample of 36 individuals from three natural populations. The markers displayed relatively high polymorphism, with the number of observed alleles per locus ranging from one to 19 (mean = 4.42). The observed and expected heterozygosity ranged from 0-1.000 and 0.083-0.882, respectively. Twenty-nine of the loci were also successfully amplified in homostylous P. sinolisteri var. aspera. Conclusions The microsatellite markers we have identified in P. sinolisteri provide powerful tools for investigating patterns of population genetic diversity and the evolutionary relationships between heterostyly and homostyly in this species.
Keyworddistyly HiSeq homostyly microsatellites Primula sinolisteri Primulaceae
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/64239
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorZhou, Wei
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Guiyang Univ Tradit Chinese Med, Dept Pharm, Guiyang 550002, Guizhou, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Plant Germplasm & Genom Ctr, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
5.Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON M5S 3B2, Canada
Recommended Citation
GB/T 7714
Wang, Xinjia,Zhong, Li,Wu, Zhikun,et al. Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(1):5.
APA Wang, Xinjia.,Zhong, Li.,Wu, Zhikun.,Sun, Huaying.,Wang, Hong.,...&Zhou, Wei.(2019).Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing.APPLICATIONS IN PLANT SCIENCES,7(1),5.
MLA Wang, Xinjia,et al."Characterization of 30 microsatellite markers in distylous Primula sinolisteri (Primulaceae) using HiSeq sequencing".APPLICATIONS IN PLANT SCIENCES 7.1(2019):5.
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