KIB OpenIR  > 中国西南野生生物种质资源库
Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining
Jiao, Si-Qian1; Sun, Yan-Qiang1; Zhang, Dong-Xu2; Gao, Qiong1; Jin, Yuqing1; Liu, Hui1; Ma, Yongpeng3; Yang, Yong4; Porth, Ilga5,6; Mao, Jian-Feng1
2019
Source PublicationAPPLICATIONS IN PLANT SCIENCES
ISSN2168-0450
Volume7Issue:1Pages:5
AbstractPremise of the Study Ephedra sinica (Ephedraceae) is a gymnosperm shrub with a wide distribution across Central and Eastern Asia. It is widely cultivated as a medicinal plant, but its wild populations are monitored to determine whether protection is needed. Methods and Results Thirty-six microsatellite markers, including 11 polymorphic markers, were developed from E. distachya RNA-Seq data deposited in the National Center for Biotechology Information dbEST database. Among 100 genotyped E. sinica individuals originating from five different population groups, the allele number ranged from three to 22 per locus. Levels of observed and expected heterozygosity ranged from 0 to 0.866 (average 0.176) and 0 to 0.876 (average 0.491), respectively. Allelic polymorphism information content ranged from 0.000 to 0.847 (average 0.333). Cross-species amplifications were successfully conducted with two related Ephedra species for all 11 di- or trinucleotide simple sequence repeats. Conclusions This study provides the first set of microsatellite markers for genetic monitoring and surveying of this medicinal plant.
KeywordEphedra sinica Ephedraceae expressed sequence tag-simple sequence repeat (EST-SSR) marker genetic diversity gymnosperm medicinal plant
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/64221
Collection中国西南野生生物种质资源库
Corresponding AuthorMao, Jian-Feng
Affiliation1.Beijing Forestry Univ, Coll Biol Sci & Technol, Minist Educ,Beijing Adv Innovat Ctr Tree Breeding, Natl Engn Lab Tree Breeding,Key Lab Genet & Breed, Beijing 100083, Peoples R China
2.Datong Univ, Coll Life Sci, Datong 037009, Shanxi, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming 650201, Yunnan, Peoples R China
4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
5.Univ Laval, Inst Syst & Integrated Biol, Pavillon Charles Eugene Marchand,1030 Ave Med, Laval, PQ G1V 0A6, Canada
6.Univ Laval, Ctr Etud Foret, 1030 Ave Med, Laval, PQ G1V 0A6, Canada
Recommended Citation
GB/T 7714
Jiao, Si-Qian,Sun, Yan-Qiang,Zhang, Dong-Xu,et al. Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(1):5.
APA Jiao, Si-Qian.,Sun, Yan-Qiang.,Zhang, Dong-Xu.,Gao, Qiong.,Jin, Yuqing.,...&Mao, Jian-Feng.(2019).Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining.APPLICATIONS IN PLANT SCIENCES,7(1),5.
MLA Jiao, Si-Qian,et al."Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining".APPLICATIONS IN PLANT SCIENCES 7.1(2019):5.
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