Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining

	Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining
	Jiao, Si-Qian 1; Sun, Yan-Qiang 1; Zhang, Dong-Xu 2; Gao, Qiong 1; Jin, Yuqing 1; Liu, Hui 1; Ma, Yongpeng 3; Yang, Yong 4; Porth, Ilga 5,6; Mao, Jian-Feng 1
	2019
发表期刊	APPLICATIONS IN PLANT SCIENCES
ISSN	2168-0450
卷号	7 期号:1 页码:5
摘要	Premise of the Study Ephedra sinica (Ephedraceae) is a gymnosperm shrub with a wide distribution across Central and Eastern Asia. It is widely cultivated as a medicinal plant, but its wild populations are monitored to determine whether protection is needed. Methods and Results Thirty-six microsatellite markers, including 11 polymorphic markers, were developed from E. distachya RNA-Seq data deposited in the National Center for Biotechology Information dbEST database. Among 100 genotyped E. sinica individuals originating from five different population groups, the allele number ranged from three to 22 per locus. Levels of observed and expected heterozygosity ranged from 0 to 0.866 (average 0.176) and 0 to 0.876 (average 0.491), respectively. Allelic polymorphism information content ranged from 0.000 to 0.847 (average 0.333). Cross-species amplifications were successfully conducted with two related Ephedra species for all 11 di- or trinucleotide simple sequence repeats. Conclusions This study provides the first set of microsatellite markers for genetic monitoring and surveying of this medicinal plant.
关键词	Ephedra sinica Ephedraceae expressed sequence tag-simple sequence repeat (EST-SSR) marker genetic diversity gymnosperm medicinal plant
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64221
专题	中国西南野生生物种质资源库
通讯作者	Mao, Jian-Feng
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Technol, Minist Educ,Beijing Adv Innovat Ctr Tree Breeding, Natl Engn Lab Tree Breeding,Key Lab Genet & Breed, Beijing 100083, Peoples R China 2.Datong Univ, Coll Life Sci, Datong 037009, Shanxi, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Univ Laval, Inst Syst & Integrated Biol, Pavillon Charles Eugene Marchand,1030 Ave Med, Laval, PQ G1V 0A6, Canada 6.Univ Laval, Ctr Etud Foret, 1030 Ave Med, Laval, PQ G1V 0A6, Canada
推荐引用方式 GB/T 7714	Jiao, Si-Qian,Sun, Yan-Qiang,Zhang, Dong-Xu,et al. Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(1):5.
APA	Jiao, Si-Qian.,Sun, Yan-Qiang.,Zhang, Dong-Xu.,Gao, Qiong.,Jin, Yuqing.,...&Mao, Jian-Feng.(2019).Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining.APPLICATIONS IN PLANT SCIENCES,7(1),5.
MLA	Jiao, Si-Qian,et al."Development of novel EST-SSR markers for Ephedra sinica (Ephedraceae) by transcriptome database mining".APPLICATIONS IN PLANT SCIENCES 7.1(2019):5.

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