| ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2 | |
Chang, Guanxiao1; Yang, Wenjuan2; Zhang, Qili2; Huang, Jinling3,4 ; Yang, Yongping1; Hu, Xiangyang1,2
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| 2019-02-01 | |
| Source Publication | PLANT PHYSIOLOGY
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| ISSN | 0032-0889 |
| Volume | 179Issue:2Pages:477-490 |
| Abstract | ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown. Here, we found that flowering time was markedly delayed and CONSTANS (CO) expression was reduced in a transgenic Arabidopsis line overexpressing AFP2 under LD conditions. Conversely, the loss-of-function afp2 mutant showed slightly earlier flowering, with higher CO expression. These data suggest that AFP2 negatively regulates photoperiod-dependent flowering time by modulating the CO signal. We then found that AFP2 exhibited circadian expression rhythms that peaked during the night. Furthermore, the C-terminus of AFP2 interacted with CO, while its N-terminal ethylene response factor-associated amphiphilic repression motif interacted with the transcriptional corepressor TOPLESS-related protein2 (TPR2). Thus, AFP2 bridges CO and TPR2 to form the CO-AFP2-TPR2 complex. Biochemical and genetic analyses showed that AFP2 mediated CO degradation during the night. AFP2 also recruited histone deacetylase activity at Flowering Locus T chromatin through its interaction with TPR2. Taken together, our results reveal an elaborate mechanism by which AFP2 modulates flowering time through coordinating the activity and stability of CO. |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.kib.ac.cn/handle/151853/64196 |
| Collection | 中国科学院东亚植物多样性与生物地理学重点实验室 |
| Corresponding Author | Hu, Xiangyang |
| Affiliation | 1.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 2.Shanghai Univ, Res Ctr Nat Prod, Shanghai Key Lab Bioenergy Crops, Plant Sci Ctr,Sch Life Sci, Shanghai 200444, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Plant Stress Biol, State Key Lab Cotton Biol, Kaifeng 475001, Peoples R China 4.East Carolina Univ, Dept Biol, Greenville, NC 27858 USA |
| Recommended Citation GB/T 7714 | Chang, Guanxiao,Yang, Wenjuan,Zhang, Qili,et al. ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2[J]. PLANT PHYSIOLOGY,2019,179(2):477-490. |
| APA | Chang, Guanxiao,Yang, Wenjuan,Zhang, Qili,Huang, Jinling,Yang, Yongping,&Hu, Xiangyang.(2019).ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2.PLANT PHYSIOLOGY,179(2),477-490. |
| MLA | Chang, Guanxiao,et al."ABI5-BINDING PROTEIN2 Coordinates CONSTANS to Delay Flowering by Recruiting the Transcriptional Corepressor TPR2".PLANT PHYSIOLOGY 179.2(2019):477-490. |
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